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100 MG
PLN 606.00
PLN 606.00
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.54
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Product Name
DNase I, grade II, from bovine pancreas
biological source
bovine pancreas
form
lyophilized
packaging
pkg of 100 mg
manufacturer/tradename
Roche
technique(s)
DNA extraction: suitable
optimum pH
~7.0
storage temp.
2-8°C
Quality Level
1 of 4
This Item | 11284932001 | D7291 | AMPD1 |
|---|---|---|---|
| technique(s) DNA extraction: suitable | technique(s) cell culture | mammalian: suitable | technique(s) DNA extraction: suitable | technique(s) RT-PCR: suitable |
| biological source bovine pancreas | biological source bovine pancreas | biological source bovine pancreas | biological source - |
| form lyophilized | form lyophilized | form buffered aqueous glycerol solution | form liquid |
| optimum pH ~7.0 | optimum pH - | optimum pH - | optimum pH - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 100 | Quality Level 100 | Quality Level 200 | Quality Level 200 |
Application
General description
Bovine pancreatic deoxyribonuclease I (DNase I) is a DNA minor grove-interacting nuclease which shows relatively low specificity. This protein is composed of two central β sheets, each composed of six β-strands. This structure is surrounded by extensive loop and α-helical regions. This enzyme shares structural similarity to exonuclease III.[5] DNase I is one of the most well characterized endonucleases of mammalian origin. It is a double-strand-specific endonuclease that requires bivalent cations for maximal activity.[6]
Other Notes
For life science research only. Not for use in diagnostic procedures.
One unit is the enzyme activity that causes an increase in the absorbance of 0.001 per minute under assay conditions.
Preparation Note
Activator: Ca2+ (0.12 mM) in combination with Mg2+ enhances activity [Laskowski (1971); Melgar and Goldthwait (1968)].
Working concentration: 1 mg/ml
Do not vortex while dissolving!
Storage conditions (working solution): Reconstituted in water, the solution can be kept for 2 to 3 days at 2 to 8 °C. Do not vortex during dissolving. Note: Dissolve at least 1 mg/ml.
Working concentration: 1 mg/ml
Do not vortex while dissolving!
Storage conditions (working solution): Reconstituted in water, the solution can be kept for 2 to 3 days at 2 to 8 °C. Do not vortex during dissolving. Note: Dissolve at least 1 mg/ml.
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
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Insulin-like growth factor binding protein 3 (IGFBP3), a hypoxia-inducible gene, regulates a variety of cellular processes including cell proliferation, senescence, apoptosis and epithelial-mesenchymal transition (EMT). IGFBP3 has been linked to the pathogenesis of cancers. Most previous studies focus upon proapoptotic
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Applied and environmental microbiology, 79(19), 5936-5941 (2013-07-23)
Human lungs are constantly exposed to bacteria in the environment, yet the prevailing dogma is that healthy lungs are sterile. DNA sequencing-based studies of pulmonary bacterial diversity challenge this notion. However, DNA-based microbial analysis currently fails to distinguish between DNA
S Cal et al.
The EMBO journal, 17(23), 7128-7138 (1998-12-08)
Bovine pancreatic deoxyribonuclease I (DNase I) is a nuclease of relatively low specificity which interacts with DNA in the minor groove. No contacts are made between the protein and the major groove of the nucleic acid. DNase I is structurally
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