ACOA-RO

Acetyl-Coenzyme A

85% (Enzymatic and Absorbance), 2% (lithium)

• Synonym(s): acetyl-coa

Properties

• description: Chemical Formula: C₂₃H₃₅N₇O₁₇P₃SLi₃
• Quality Level: 100
• assay: 2% (lithium); 85% (Enzymatic and Absorbance)
• form: solid
• mol wt: M_r 809.6 (acetyl-CoA); M_r 827.4 (acetyl-CoA-Li₃)
• packaging: pkg of 010 mg (10101893001); pkg of 200 mg (11585371001); pkg of 050 mg (10101907001)
• manufacturer/tradename: Roche
• storage temp.: 2-8°C

Description

General description

Acetyl-Coenzyme A, trilithium salt

Application

Acetyl-Coenzyme A is used to assay CAT enzyme activity in cell extracts using radioisotopes. CAT enzyme activity in cell extracts catalyzes the transfer of acetyl groups from acetylcoenzyme A to chloramphenicol. A number of assays have been developed to measure CAT activity in cell extracts. Acetyl-Coenzyme A has also been used to determine citrate synthase activity.

Packaging

10, 50, 200 mg

Biochem/physiol Actions

Acetyl-Coenzyme A (Ac-CoA) is the end product of glycolysis and takes part in the Ac-CoA pathway, which is a metabolic pathway for carbon compounds. Ac-CoA is important in cholesterol synthesis. It also takes part in fatty acid biosynthesis and catabolism of polyamines like spermine and spermidine. A low level of Ac-CoA leads to a loss in glial cell and neuronal function. Ketone bodies and triglycerides give rise to Ac-CoA on hydrolysis and this indirectly leads to increased histone acetylation.

Preparation Note

Working solution: Optimal solvent: water or aqueous solution with a weak acidic pH (4 to 5).
Storage conditions (working solution): -15 to -25 °C
50 mg/ml in phosphate buffer, pH 7, is stable for 3 weeks at -15 to -25 °C. Unfrozen solutions should be immediately used.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable