RSTREP-RO

Streptavidin, recombinant

lyophilized, ≥17 units/mg protein, pH range 5.0-9.0

• Synonym(s): streptavidin

Properties

• form: lyophilized
• Quality Level: 100
• specific activity: ≥17 units/mg protein
• mol wt: ~52 kDa
• packaging: pkg of 1 mg (11721666001); pkg of 5 mg (11721674001)
• manufacturer/tradename: Roche
• pH range: 5.0-9.0
• pI: 7.4-7.7 (two main bands)
• solubility: water: 10 mg/L (mA₄₀₅=100)
• storage temp.: 2-8°C

Description

Application

Use Streptavidin, recombinant for coating of solid phases (e.g., microplates, beads etc.) as well with biotin-labeled haptens and antibodies in universal immunological detection systems (streptavidin/biotin system).

Packaging

1, 5 mg

Sequence

Streptavidin is a tetramer with a molecular weight of approximately 53 kD consisting of four subunits of approximately 13 kD, each containing a single biotin-binding site.
Each subunit has six tyrosine residues.The protein is free of carbohydrate.

Unit Definition

One unit binds 1 μg D-biotin at pH 8.9.

Physical form

White lyophilizate; lyophilized in a 20 mM potassium phosphate, pH 6.5 solution
Protein: 0.7 ± 0.1mg protein lyophilizate (A₂₈₂ = 3.1)
K_D: 10^-15M

Reconstitution

Add 1 ml double-distilled water to get a final concentration of 1 mg/ml (11 721 666 001) or 5 mg/ml (11 721 674 001), respectively.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): does not flash
• Flash Point(C): does not flash