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MRS Agar

Name: MRS Agar
Brand: SIAL
Price: 788 PLN

NutriSelect^® Basic, powder, nonselective for Lactobacillus Spp., pack of 500 g

Synonym(s):

Lactobacillus Agar according to DeMan, Rogosa and Sharpe, Lactobacillus Agar according to DeMan, Rogosa, and Sharpe

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Pack Size	SKU	Availability	Price
500 g		Please contact Customer Service for Availability	PLN 788.00

About This Item

NACRES:

NA.74

UNSPSC Code:

41171605

Manufacturer/tradename:

NutriSelect^® Basic

Form:

powder

Suitability:

nonselective for Lactobacillus Spp., selective by low pH for Bifidobacterium spp.

Application(s):

agriculture
bioburden testing
environmental
food and beverages
microbiology

PLN 788.00

Please contact Customer Service for Availability

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Properties

Quality Level

100

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 12 g/L , dipotassium hydrogen phosphate, 2 g/L , D(+)-glucose, 20 g/L , magnesium sulfate (equivalent to 0.2 g/lheptahydrate), 0.1 g/L , manganous sulfate monhydrate, 0.05 g/L , meat extract, 5 g/L , sodium acetate, 5 g/L , triammonium citrate, 2 g/L , universal peptone, 10 g/L , yeast extract, 5 g/L

packaging

pack of 500 g

manufacturer/tradename

NutriSelect^® Basic

technique(s)

microbiological culture: suitable

final pH

6.5±0.2 (25 °C)

application(s)

agriculture
bioburden testing
environmental
food and beverages
microbiology

suitability

nonselective for Lactobacillus Spp., selective by low pH for Bifidobacterium spp.

Description

General description

MRS Agar, also known as DeMan-Rogosa-Sharpe Agar, is a microbiological medium specifically designed for the enrichment, cultivation, and isolation of Lactobacillus species from various materials. It is a non-selective medium with a low pH that favors the growth of Lactobacillus spp. and Bifidobacterium spp. The medium contains essential nutrients such as glucose, meat extract, yeast extract, and peptones, which provide nitrogenous and carbonaceous compounds, amino acids, and vitamins necessary for microbial growth. Sodium acetate and ammonium citrate enhance selectivity by inhibiting the growth of unwanted organisms. The media contains polysorbate (Tween 80), acetate, magnesium, and manganese, which are known to act as specific growth factors for Lactobacilli, and provide a rich nutrient base.

Application

MRS Agar is widely used for the detection, isolation, and cultivation of Lactobacillus spp. and other lactic acid bacteria from food, beverages, and environmental samples. It is applied in agriculture, food and beverage quality control, environmental monitoring, and microbiology research. The medium supports the growth of fastidious Lactobacillus species, such as Lactobacillus brevis and Lactobacillus fermentum, and is often used in CO₂-enriched atmospheres to enhance growth.

Features and Benefits

NutriSelect^® Basic is a powdered culture media offering

Quality control with growth promotion test according to industry standards
Quality assurance acc. to ISO 9001
Cost-efficient powdered media

Preparation Note

Suspend 61.15 g in 1000 ml distilled water containing 1 ml polysorbate 80 (TWEEN^® 80; Sigma P8074). Boil to dissolve the medium completely and mix thoroughly. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. If necessary adjust the pH with glacial acetic acid after sterilization.

Other Notes

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

TWEEN is a registered trademark of Croda International PLC

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This Item	41782	17154	45183
Millipore 69964 MRS Agar	Millipore 41782 MRS Agar	Millipore 17154 Lactobacillus bulgaricus Agar (Base)	Millipore 45183 Enterococcus Selective Agar
form powder	form powder	form powder	form powder
suitability nonselective for Lactobacillus Spp., selective by low pH for Bifidobacterium spp.	suitability selective and differential for Lactobacillus spp., selective by low pH for Bifidobacterium spp.	suitability selective by low pH for Lactobacillus spp.	suitability Enterococcus spp., selective and differential for Streptococcus spp.
manufacturer/tradename NutriSelect^® Basic	manufacturer/tradename NutriSelect^® Plus	manufacturer/tradename NutriSelect^® Plus	manufacturer/tradename NutriSelect^® Basic
application(s) agriculture bioburden testing environmental food and beverages microbiology	application(s) agriculture bioburden testing environmental food and beverages, microbiology	application(s) environmental food and beverages, microbiology	application(s) bioburden testing clinical testing environmental food and beverages water monitoring, microbiology
technique(s) microbiological culture: suitable	technique(s) microbiological culture: suitable	technique(s) microbiological culture: suitable	technique(s) microbiological culture: suitable
composition agar, 12 g/L , D(+)-glucose, 20 g/L , manganous sulfate monhydrate, 0.05 g/L , sodium acetate, 5 g/L , universal peptone, 10 g/L , dipotassium hydrogen phosphate, 2 g/L , meat extract, 5 g/L , yeast extract, 5 g/L , magnesium sulfate (equivalent to 0.2 g/lheptahydrate), 0.1 g/L , triammonium citrate, 2 g/L	composition agar, 15.0 g/L , ammonium citrate, 2.0 g/L , bromocresol green, 0.04 g/L , captan, 0.004 g/L , dextrose, 20.0 g/L , dipotassium phosphate, 2.0 g/L , magnesium sulfate, 0.1 g/L , manganese sulfate, 0.05 g/L , 2-phenylethyl alcohol, 3.0 g/L , proteose peptone (vegetable), 10.0 g/L , sodium acetate, 5.0 g/L , yeast extract, 5.0 g/L	composition agar, 20 g/L , beef extract, 10 g/L , casein enzymic hydrolysate, 10 g/L , dextrose, 20 g/L , dipotassium phosphate, 2 g/L , polysorbate 80, 1 g/L , tomato juice, 2 g/L , yeast extract, 5 g/L	composition agar, 10 g/L , disodium hydrogen phosphate monohydrate, 4 g/L , D(+)-glucose, 2 g/L , sodium azide, 0.4 g/L , 2,3,5-triphenyltetrazolium chloride, 0.1 g/L , tryptose, 20 g/L , yeast extract, 5 g/L