N4638

Neutral Red powder, BioReagent, cell culture | mammalian | 553-24-2

BioReagent, Powder

• Synonym(s): Neutral Red, 3-Amino-7-dimethylamino-2-methylphenazine hydrochloride, Basic Red 5, Toluylene red
• Empirical Formula (Hill Notation): C₁₅H₁₇ClN₄
• CAS Number: 553-24-2
• Molecular Weight: 288.78
• UNSPSC Code: 12171500
• NACRES: NA.47
• PubChem Substance ID: 24897707
• EC Number: 209-035-8
• MDL number: MFCD00012651
• Colour Index Number: 50040
• Beilstein/REAXYS Number: 3918740

Properties

• Product Name: Neutral Red powder, BioReagent, cell culture | mammalian | 553-24-2, powder, BioReagent, suitable for cell culture
• InChI: 1S/C15H16N4.ClH/c1-9-6-13-15(8-11(9)16)18-14-7-10(19(2)3)4-5-12(14)17-13;/h4-8H,16H2,1-3H3;1H
• InChI key: PGSADBUBUOPOJS-UHFFFAOYSA-N
• SMILES string: Cl.CN(C)c1ccc2nc3cc(C)c(N)cc3nc2c1
• product line: BioReagent
• form: powder
• composition: Dye content, ≥90%
• technique(s): cell culture | mammalian: suitable; titration: suitable
• color: brown to black
• pH: 6.8-8.0, red to yellow
• mp: 290 °C (dec.) (lit.)
• solubility: H₂O: 10 mg/mL
• application(s): diagnostic assay manufacturing
• storage temp.: 2-8°C
• Quality Level: 200

Description

Application

Neutral red has been used in several histological staining applications that include:

• as a nuclear stain
• as a counterstain in the Gram procedure, either alone, or as a component of the Twort stain, a fast green-neutral red mixture.
• fluorescent staining of nucleic acids in nervous tissue, of lipids and hydrophobic substances such as suberin and of phenolic compounds in algal cell walls
• detection of viable yeast cells in dandruff samples and of mussel larvae
• Neutral red has also been used as a stain in flow cytometry.

Useful as an indicator for preparing neutral red paper, and as a biological stain.

General description

Neutral red (C.l. 50040, Cl Basic red 5) is a protonated diaminophenazine dye that has a small planar conjugated system.

Preparation Note

Purified

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)