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A3151

Avidin–Peroxidase

lyophilized powder

Synonym(s):

Avidin-POD

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Size/SKUAvailabilityPrice
1 mg
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606,00 PLN
2 mg
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1.020,00 PLN
5 mg
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2.180,00 PLN

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00130587
Form:
lyophilized powder
Biological source:
avidin from egg white, enzyme from horseradish

606,00 PLN

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Quality Segment

biological source

avidin from egg white, enzyme from horseradish

conjugate

peroxidase conjugate

form

lyophilized powder

composition

Protein, ≥70% E1%/280

extent of labeling

0.7-2.0 mol peroxidase per mol avidin

technique(s)

direct ELISA: 1:50,000

solubility

H2O: soluble 1 mg/mL, PBS: soluble

storage temp.

−20°C

General description

Avidin is homotetrameric protein (68kDa) that is obtained from egg whites. This egg protein binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.

Application

Avidin-Peroxidase has been used for detecting anti-5-bromodeoxyuridine (anti-BrdU) binding and OVA-IgG1.
To detect Ga1-deficient IgA1 antibodies in mouse serum, ELISA assays were performed using sera which was incubated with biotinylated HAA, which binds IgA1, in 96-well plates. HRP-conjugated avidin was then incubated with the sera for 40 minutes at 37 degrees.

Physical form

Lyophilized powder containing citrate buffer.

Preparation Note

Labeled with Type VI peroxidase. Coupled by a modification of the method of Sullivan, M.J., et al., FEBS Lett., 95, 311 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item
A7294A2901A9207
Avidin–Peroxidase lyophilized powder

Sigma-Aldrich

A3151

Avidin–Peroxidase

Avidin–Alkaline Phosphatase buffered aqueous solution

Sigma-Aldrich

A7294

Avidin–Alkaline Phosphatase

Avidin–FITC from egg white lyophilized powder

Sigma-Aldrich

A2901

Avidin–FITC from egg white

Avidin−Agarose from egg white aqueous glycerol suspension

Sigma-Aldrich

A9207

Avidin−Agarose from egg white

description

lyophilized powder

description

buffered aqueous solution

description

lyophilized powder

description

aqueous glycerol suspension

biological source

enzyme from horseradish, avidin from egg white

biological source

enzyme from bovine (calf) intestine, avidin from egg white

biological source

-

biological source

egg white

technique(s)

direct ELISA: 1:50,000

technique(s)

western blot: 1:150,000-1:300,000 using using β-actin in total cell extract of HeLa cells (5-10 μg per lane, direct ELISA: 1:70,000

technique(s)

immunohistochemistry: suitable

technique(s)

-

form

lyophilized powder

form

buffered aqueous solution

form

lyophilized powder

form

aqueous glycerol suspension

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

solubility

H2O: soluble 1 mg/mL, PBS: soluble

solubility

-

solubility

-

solubility

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

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Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Codes

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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