A3543

Anti-Human IgG (whole molecule)−Agarose antibody produced in goat

IgG fraction of antiserum, PBS suspension

• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00162452
• Conjugate: agarose conjugate
• Clone: polyclonal
• Application: IEP
• Citations: 8

Properties

• biological source: goat
• Quality Segment: 200
• conjugate: agarose conjugate
• antibody form: IgG fraction of antiserum
• antibody product type: secondary antibodies
• clone: polyclonal
• form: PBS suspension
• species reactivity: human
• extent of labeling: ~2.4 mg Ab per mL
• technique(s): immunoelectrophoresis: suitable
• capacity: 1 mg/mL binding capacity
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections.

Immunogen

Pooled normal human serum

Application

Anti-human IgG (whole molecule) is suitable for immunoelectrophoresis. Antibody depletion from normal human serum was carried out by treating diluted serum with 50 microl of agarose-linked goat anti-human IgG (A3543) for 90 minutes at 4 °.

Antibody depletion from normal human serum was carried out by treating diluted serum with 50 μl of agarose-linked goat anti-human IgG (A3543) for 90 minutes at 4 degrees.

Biochem/physiol Actions

Anti-human IgG recognizes human IgG (whole molecules). Goat anti-human is purified by ion exchange chromatography and then covalently bound to agarose.

IgG stimulates the classical pathway of the complement system. It neutralizes virus particles and toxins. IgG plays a crucial role in antibody-dependent cell-mediated cytotoxicity (ADCC). IgG might be associated with allergy. It has the longest serum half life.. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections.

Physical form

Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable