For catalog number A8181, we have not studied storage after being diluted with water. We have on the undiluted product a "one year guarantee of performance" for this product from date of shipping to customers.
A8181
Amido Black Staining Solution 2X
electrophoresis reagent
Synonym(s):
Naphthol Blue Black solution, Amido Black 10B
Sign In to View Organizational & Contract Pricing.
Select a Size
1 EA
PLN 541.00
PLN 541.00
Please contact Customer Service for Availability
About This Item
Empirical Formula (Hill Notation):
C22H14N6Na2O9S2
CAS Number:
Molecular Weight:
616.49
UNSPSC Code:
12171500
NACRES:
NA.32
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
5374263
Skip To
Product Name
Amido Black Staining Solution 2X, electrophoresis reagent
InChI
1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2/b26-25+,27-24+;;
SMILES string
[Na+].[Na+].Nc1c(\N=N/c2ccc(cc2)[N+]([O-])=O)c(cc3cc(c(\C=C/c4ccccc4)c(O)c13)S([O-])(=O)=O)S([O-])(=O)=O
InChI key
AOMZHDJXSYHPKS-DROYEMJCSA-L
form
liquid
Quality Level
Looking for similar products? Visit Product Comparison Guide
Related Categories
1 of 4
This Item | N3393 | N9002 | 199664 |
|---|---|---|---|
| form liquid | form powder | form powder | form powder |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Application
General description
Amido black (AB) is an acidic dye used for staining proteins.[4] It can detect proteins levels of 50ng in both polyvinylidene difluoride (PVDF) and nitrocellulose membrane.[5] AB aids protein visualization even at low concentrations.[6] It has applications in forensics due to its ability to stain blood proteins in fingerprints.[4]
Preparation Note
After dilution with deionized water to a final volume of 500 ml, solution will contain 0.1% (w/v) amido black, 25% (v/v) isopropanol and 10% (v/v) acetic acid.
related product
Product No.
Description
Pricing
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Central nervous system
Storage Class
3 - Flammable liquids
wgk
WGK 1
flash_point_f
53.6 °F
flash_point_c
12 °C
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Detection of proteins on blot membranes
Goldman A, et al.
Current Protocols in Protein Science / Editorial Board, John E. Coligan ... [Et Al.], 86(1), 10-18 (2016)
Placental release of taurine to both the maternal and fetal circulations in human term pregnancies
Holm MB, et al.
Amino Acids, 50(9), 1205-1214 (2018)
Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to a positively charged membrane filter.
J M Gershoni et al.
Analytical biochemistry, 124(2), 396-405 (1982-08-01)
Protein Blotting and detection: methods and protocols, 86(1), 10-18 (2009)
Meghan C Hughes et al.
The Journal of physiology, 598(7), 1377-1392 (2019-01-24)
Ninety-eight per cent of patients with Duchenne muscular dystrophy (DMD) develop cardiomyopathy, with 40% developing heart failure. While increased propensity for mitochondrial induction of cell death has been observed in left ventricle, it remains unknown whether this is linked to
Articles
Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.
Related Content
Datasheet
-
can this reagent be stored for an extended period of time after being diluted to 1x with water?
1 answer-
Helpful?
-
-
Could you please provide the staining procedure and destaining formulation for the Amino Black Staining solution 2x that is suitable for staining in 2-40% gels?
1 answer-
To detect total protein on a Western blot, follow these steps:
1.Prepare Amido Black 1X Staining Solution by diluting Amido Black Staining Solution (Product Number A8181) 1:1 with deionized water.
2. After transferring proteins onto nitrocellulose membrane, immerse membrane in sufficient Amido Black 1X Staining Solution to cover. Stain for 1 minute.
3. Destain the membrane by placing in an aqueous solution containing 25% isopropanol and 10% acetic acid for 30 minutes. If a lighter background is desired, destain overnight.
4. Store the destained membrane in deionized water or allow to air dry.
Product Number A8181 Store at Room Temperature Product Description: Amido Black staining solution is used for the detection of microgram quantities of proteins transferred to nitrocellulose membranes. In comparison to staining with Brilliant Blue R, staining with Amido Black results in significantly less background on nitrocellulose membranes. Because nitrocellulose membranes are more stable in isopropanol than in methanol or other solvents, the formulation contains isopropanol. After a 1:1 dilution with deionized water, Amido Black Staining Solution contains: 0.1% Amido Black (w/v), 25% Isopropanol (v/v), 10% Acetic Acid (v/v). Note: Not suitable for use with charge modified nylon membranes.Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service