Merck
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B3934

Supelco

Biuret reagent

protein detection level 150-1,000 μg/mL

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Synonym(s):
Micro Lowry, Onishi & Barr Modification
PubChem Substance ID:
24891740
NACRES:
NA.32

form

liquid

Quality Level

100

protein detection level measuring range

150-1,000 μg/mL

storage temp.

2-8°C

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B69163944230891
Supelco

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B3934

Biuret reagent

Supelco

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B6916

Bradford Reagent

Millipore

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39442

Barritt′s Reagent B

Biliverdin hydrochloride ≥97.0% (TLC)

Sigma-Aldrich

30891

Biliverdin hydrochloride

protein detection level measuring range

150-1,000 μg/mL

protein detection level measuring range

-

protein detection level measuring range

-

protein detection level measuring range

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

storage temp.

−20°C

Quality Level

100

Quality Level

100, 200

Quality Level

200

Quality Level

100

General description

Biuret reagent is used for measuring total protein concentration. It comprises of copper II (cupric ion) alkaline solution, which interacts with the peptide bond to form a blue adduct which is measured at 540nm. Biuret method is less sensitive when compared to other protein estimation methods like Lowry and Folin-Ciocalteu techniques.

Application

Biuret reagent has been used for protein quantification of high-pressure thermal hydrolysis lysate(HPTH), oyster hemolymph and Clostridium cellulovorans (CBDclos) aggregates.

related product

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Morgan-Sagastume F, et al.
Bioresource Technology, 102(3), 3089-3097 (2011)
Non-lethal determination of sex and reproductive condition of Eastern oysters Crassostrea virginica Gmelin using protein profiles of hemolymph by Proteinchip textregistered and SELDI-TOF-MS technology
Li Y, et al.
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Proteins and Albumin
Roger LB
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Bacterial inclusion bodies as potential synthetic devices for pathogen recognition and a therapeutic substance release
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Microbial cell factories, 12(1), 16-16 (2013)
A simplified method of quantitating protein using the biuret and phenol reagents.
S T Ohnishi et al.
Analytical biochemistry, 86(1), 193-200 (1978-05-01)

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