The product is a buffered aqueous solution, not a lyophilized powder, so it does not require reconstitution. The solution contains Cathepsin B at a specific protein concentration and activity, both of which are lot-dependent and provided on the CoA.
C8571
Cathepsin B from human liver
buffered aqueous solution, ≥200 units/mg protein (One unit is defined as the amount of enzyme that hydrolyzes 1 μmol of Z-Arg-Arg-beta-naphthylamide per minute at 40 °C, using 100 mM Na/K phosphate, pH 6.0, with 1.33 mM EDTA and 2 mM DTT as the activation buffer.)
Synonym(s):
Cathepsin B1
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| Size/SKU | Availability | Price |
|---|---|---|
25 μg | Please contact Customer Service for Availability | 6.760,00 PLN |
About This Item
CAS Number:
UNSPSC Code:
12352200
NACRES:
NA.32
EC Number:
MDL number:
Specific activity:
≥200 units/mg protein (One unit is defined as the amount of enzyme that hydrolyzes 1 μmol of Z-Arg-Arg-beta-naphthylamide per minute at 40 °C, using 100 mM Na/K phosphate, pH 6.0, with 1.33 mM EDTA and 2 mM DTT as the activation buffer.)
Biological source:
human liver
biological source
human liver
Quality Segment
form
buffered aqueous solution
specific activity
≥200 units/mg protein (One unit is defined as the amount of enzyme that hydrolyzes 1 μmol of Z-Arg-Arg-beta-naphthylamide per minute at 40 °C, using 100 mM Na/K phosphate, pH 6.0, with 1.33 mM EDTA and 2 mM DTT as the activation buffer.)
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... CTSB(1508)
General description
Cathepsin B gene is mapped to human chromosome 8p23.1. It is a lysosomal cysteine with 252 amino acids distributed in two chains and is homologous to rat liver sequence. It has disc shaped structure with two domains namely the left-hand ′L′ domain at the amino terminal and right hand ′R′ domain at the carboxyl-terminal.
Application
Cathepsin B from human liver has been used:
- as a positive control in the screening Cathepsin B inhibitor assay
- in wall extension (expansin activity) assay
- in microinjection human foreskin fibroblasts to test its effect on apoptosis induction
Biochem/physiol Actions
Cathepsin B from human liver is highly expressed in the esophageal adenocarcinoma (EAC). It reduces hepatic inflammation and fibrogenesis and may serve as a potential candidate for treating liver diseases.
Cathepsin B has been found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells. Translocation of cathepsin B from the cytoplasm to the nucleus contributes to bile salt induced apoptosis of rat hepatocytes. Levels of cathepsin B in PC12 cells significantly decrease 12 to 24 hours after apoptosis is induced.
Found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells.
Packaging
Package size based on protein content
Physical form
Solution in 50 mM sodium acetate, pH 5.0, with 1 mM EDTA.
Other Notes
One unit will liberate 1 nanomole of 7-amino-4-methylcoumarin from Z-Arg-Arg 7-amido-4-methylcoumarin per min at pH 6.0 at 40 °C.
1 of 1
This Item | |||
|---|---|---|---|
| description buffered aqueous solution, ≥200 units/mg protein (One unit is defined as the amount of enzyme that hydrolyzes 1 μmol of Z-Arg-Arg-beta-naphthylamide per minute at 40 °C, using 100 mM Na/K phosphate, pH 6.0, with 1.33 mM EDTA and 2 mM DTT as the activation buffer.) | description lyophilized powder, ≥5 units/mg protein | description Cathepsin B, Human Liver, CAS 9047-22-7, is a purified native cathepsin B from human liver, purified by affinity chromatography. Upregulated in many types of tumors. | description - |
| specific activity ≥200 units/mg protein (One unit is defined as the amount of enzyme that hydrolyzes 1 μmol of Z-Arg-Arg-beta-naphthylamide per minute at 40 °C, using 100 mM Na/K phosphate, pH 6.0, with 1.33 mM EDTA and 2 mM DTT as the activation buffer.) | specific activity ≥5 units/mg protein | specific activity ≥10 units/mg protein | specific activity - |
| biological source human liver | biological source - | biological source human liver | biological source rabbit |
| Gene Information human ... CTSB(1508) | Gene Information human ... CTSB(1508) | Gene Information human ... CTSB(1508) | Gene Information human ... CTSB(1508) |
| form buffered aqueous solution | form lyophilized powder | form liquid | form liquid |
| storage temp. −70°C | storage temp. −20°C | storage temp. −70°C | storage temp. −20°C |
| shipped in dry ice | shipped in - | shipped in wet ice | shipped in wet ice |
Storage Class
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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how to reconstitute the cathepsin B? If it is written >1500units/mg, and C8571-25UG, how come it is in aqeous solution?
1 answer-
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