Merck
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CASP3F

Sigma-Aldrich

Caspase 3 Assay Kit, Fluorimetric

EC Number:
NACRES:
NA.84

usage

sufficient for 1,000 multiwell tests
sufficient for 100 standard tests (2 ml)

Quality Level

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CASP3(836)

General description

The Caspase 3 Fluorometric Assay Kit is based on the hydrolysis of acetyl Asp-Glu-Val-Asp 7-amido-4-methylcoumarin (Ac-DEVD-AMC) by caspase 3, resulting in the release of the fluorescent 7-amino-4-methylcoumarin (AMC). The excitation and emission wavelengths of AMC are 360 nm and 460 nm respectively. The concentration of the AMC released can be calculated from a calibration curve prepared with AMC standards (AMC standard included with the kit).

Application

The Caspase 3 Fluorimetric Assay Kit is designed for the detection and measurement of caspase 3 activity in cell extracts or purified preparations of caspase 3.

Unit Definition

One unit of caspase 3 will cleave 1.0 μmol of Ac-DEVD per min at pH 7.4 at 25 °C.

Kit Components Only

Product No.
Description

  • Water (17-18 megaOhm) 125 mL

Kit Components Also Available Separately

Product No.
Description
SDS

  • A1086Ac-DEVD-AMC substrate 2.5 mg

  • A0835Ac-DEVD-CHO inhibitor .5 mg

  • A98917-Amino-4-methylcoumarin standard 1 mg

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

12 - Non Combustible Liquids

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

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