L5178Y

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514
• Biological source: mouse thymus
• Relevant disease(s): cancer
• Growth mode: Suspension
• Karyotype: Not specified
• Morphology: Lymphoblast
• Manufacturer/tradename: ECACC

Properties

• biological source: mouse thymus
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 87111908-DNA-5UG, pkg of vial of cells 87111908-1VL
• growth mode: Suspension
• karyotype: Not specified
• morphology: Lymphoblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Application

Toxicity screening and radiation studies

Biochem/physiol Actions

Derived from a thymic tumour induced in a DBA/2 mouse by methylcholanthrene. The cells were adapted for in vitro growth. The cells have been widely used in toxicity and radiation studies.

Mouse DBA/2 lymphoma

Preparation Note

Fischer′s + 2mM Glutamine + 1% Sodium Pyruvate (NaP) + 10% Horse Serum (HS) or RPMI 1640 + 10% Foetal Bovine Serum (FBS).

Maintain cultures between 3x10,000 to 5x100,000 cells/ml; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.