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CS0010

β-Secretase (BACE1) Activity Detection Kit (Fluorescent)

1 kit sufficient for 250 reactions

Synonym(s):

BACE1 Activity Detection Kit (Fluorescent)

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1 kit
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5.710,00 PLN

About This Item

NACRES:
NA.84
UNSPSC Code:
12161503

5.710,00 PLN

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Quality Segment

300

usage

 kit sufficient for 250 reactions

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... BACE1(23621)

Application

The kit provides all the reagents required for an efficient detection of BACE1 activity. It contains an enzyme to be used for screening for potential BACE1 inhibitors. The assay is based on the fluorescence resonance energy transfer (FRET) method in which the fluorescence signal enhancement is observed after substrate cleavage by BACE1.

Biochem/physiol Actions

BACE1 is a transmembrane protease responsible for the β site cleavage of the amyloid precursor protein (APP) to produce amyloid β peptide (Aβ). The accumulation of Aβ in the brain is a primary cause for the progression of Alzheimer′s. BACE1 is a target for inhibitor drug discovery.

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This Item
S4195RAB0623AB5832
β-Secretase (BACE1) Activity Detection Kit (Fluorescent) 1 kit sufficient for 250 reactions

Sigma-Aldrich

CS0010

β-Secretase (BACE1) Activity Detection Kit (Fluorescent)

β-Secretase human recombinant, expressed in HEK 293 cells (C-terminal FLAG tagged), ≥10,000 units/mg protein

Sigma-Aldrich

S4195

β-Secretase human

Human BACE1 / Beta-secretase 1 ELISA Kit

Sigma-Aldrich

RAB0623

Human BACE1 / Beta-secretase 1 ELISA Kit

Anti-BACE Antibody Chemicon®, from rabbit

Sigma-Aldrich

AB5832

Anti-BACE Antibody

description

1 kit sufficient for 250 reactions

description

recombinant, expressed in HEK 293 cells (C-terminal FLAG tagged), ≥10,000 units/mg protein

description

-

description

Chemicon®, from rabbit

Quality Level

300

Quality Level

200

Quality Level

-

Quality Level

100

usage

 kit sufficient for 250 reactions

usage

-

usage

-

usage

-

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

-

Gene Information

human ... BACE1(23621)

Gene Information

human ... BACE1(23621)

Gene Information

human ... BACE1(23621)

Gene Information

human ... BACE1(23621)

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

Kit Components Only

Product No.
Description
  • Fluorescent Assay Buffer 50 mL
  • Stop Solution 15 mL
  • Substrate (MOCA-SEV-NL-DAEFR-DNP-RR) 500 μL
  • Assay Standard 140 μL
  • BACE1 (β−Secretase) 300 units 100 μL

Storage Class

10 - Combustible liquids

wgk

WGK 3

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Questions

1–2 of 2 Questions  

  1. What is the PDB ID of enzyme used?

    1 answer

    1. This kit includes human BACE1/?-secretase enzyme for activity detection and inhibitor screening. The exact PDB ID for the enzyme is not specified, however the protein is identical to product S4195, UniProt P56817. Please see the links below to review information that may be helpful.

      UniProt:
      https://www.uniprot.org/uniprotkb/P56817/entry

      RCSB PDB page for UniProt P56817
      https://www.rcsb.org/groups/sequence/polymer_entity/P56817

      Also note that commonly cited human BACE1 structures include PDB IDs 2ZHT and 1W50, References:
      PDB ID 2ZHT: Human BACE1 crystal structure — https://www.rcsb.org/structure/2ZHT
      PDB ID 1W50: Human BACE apo structure — https://www.rcsb.org/structure/1W50

      Helpful?

  2. what is the concentration of BACE1 enzyme in micromolar?

    1 answer

    1. The B9059 BACE-1 product included in the kit, serves as a positive control. The Technical Bulletin notes that the concentration of this item is approximate 3 units/microliter. This material has not been tested for purity. Therefore, a molar concentration cannot be provided.

      Helpful?

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