Merck
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D7290

Sigma-Aldrich

Deoxyribonucleic acid from herring sperm

single-stranded for hybridization

Synonym(s):
DNA
CAS Number:
EC Number:
MDL number:
eCl@ss:
32160414
NACRES:
NA.52

grade

for molecular biology

Quality Level

form

solution

shipped in

dry ice

storage temp.

−20°C

General description

Suitable for use as a blocking agent in Southern hybridizations.

Application

Deoxyribonucleic acid from herring sperm is suitable for use as a blocking agent in Southern hybridizations. It was used in preparation of brain sections of mice for BrdU immunohistochemistry.[1] It was also used for hybridization reaction of dot blot membranes.[2]
Many factors contribute to the signal-to-noise ratio in nucleic acid hybridizations. These factors include the presence of solvent (formamide), hybridization temperature, length of hybridization, volume of hybridization solution, degree and method of agitation, use of blocking reagents, concentration and specific activity of the probe, use of molecular agents to increase the rate of nucleic acid reassociation, and the degree of stringency used during the washing of the membrane.

In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques.

Preparation Note

This DNA is phenol-chloroform extracted, ethanol precipitated, and sonicated to produce single-stranded fragments which comigrate with the 587 and 831 base pair marker fragments.

Other Notes

DNA in solution will reanneal on standing at room temperature so it is recommended to boil the solution for 10 minutes and then cool on ice for at least 5 minutes prior to use.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

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