This product can be resuspended in pH 8 Tris buffer, as mentioned on the Enzymatic Assay of Elastase webpage. For more details, please refer to the following link: https://www.sigmaaldrich.com/technical-documents/protocol/protein-biology/enzyme-activity-assays/enzymatic-assay-of-elastase#preparation
E0258
Elastase from porcine pancreas
Type IV, Protein 50-90 %, lyophilized powder, ≥4.0 units/mg protein (biuret)
Synonym(s):
Elastase from hog pancreas, Pancreatopeptidase E
Sign In to View Organizational & Contract Pricing.
Select a Size
1 MG
PLN 260.00
5 MG
PLN 843.00
10 MG
PLN 1,320.00
20 MG
PLN 2,300.00
50 MG
PLN 4,550.00
PLN 260.00
Please contact Customer Service for Availability
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
254-453-6
MDL number:
Skip To
Product Name
Elastase from porcine pancreas, Type IV, Protein 50-90 %, lyophilized powder, ≥4.0 units/mg protein (biuret)
biological source
Porcine pancreas
type
Type IV
form
lyophilized powder
specific activity
≥4.0 units/mg protein (biuret)
composition
Protein, 50-90%
foreign activity
trypsin ≤50 BAEE units/mg protein
storage temp.
−20°C
Quality Level
Looking for similar products? Visit Product Comparison Guide
Related Categories
1 of 4
This Item | E0127 | E1250 | E7885 |
|---|---|---|---|
| specific activity ≥4.0 units/mg protein (biuret) | specific activity ≥4.0 units/mg protein | specific activity ≥4.0 units/mg protein | specific activity ≥4 units/mg protein |
| biological source Porcine pancreas | biological source - | biological source Porcine pancreas | biological source Porcine |
| form lyophilized powder | form lyophilized powder | form suspension | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| foreign activity trypsin ≤50 BAEE units/mg protein | foreign activity - | foreign activity trypsin ≤50 BAEE units/mg protein | foreign activity Trypsin ≤50 BAEE units/mg protein |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Application
Elastase from Sigma has been used to examine the extent of proteolytic degradation of BSA that is treated with hydroxyl radical.[1] It has also been used to purify elastase-inhibitory lipid derivative from a cyanobacterium, Microcystis Ku2.[2]
Elastase from porcine pancreas has been used in a study to assess the molecular bases for human leucocyte elastase inhibition. Elastase from porcine pancreas has also been used in a study to investigate the molecular cloning and expression of serum calcium-decreasing factor (caldecrin).
Biochem/physiol Actions
Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.
Elastase is a serine protease with broad specificity as it cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val and Leu.[7] The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester. The pH optimum is found to be 8.0-8.5. It does not require any activator, but it is inhibited by diisopropyl fluorophosphate, phenylmethanesulfonyl fluoride, α2-macroglobulin, α1-antitrypsin, sulfonyl fluorides and p-dinitrophenyl diethylphosphate and high salt concentrations. It is extensively used in tissue and cell dissociation procedures. Elastase is effective in the isolation of Type II lung cells. Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.[8]
Elastase is a serine protease with broad specificity as it cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val, and Leu. The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester. The pH optimum is found to be 8.0-8.5. It does not require any activator, but it is inhibited by diisopropyl fluorophosphate, phenylmethanesulfonyl fluoride, α2-macroglobulin, α1-antitrypsin, sulfonyl fluorides and p-dinitrophenyl diethylphosphate and high salt concentrations. It is extensively used in tissue and cell dissociation procedures. Elastase is effective in the isolation of Type II lung cells.
General description
Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges.[9] The molecular mass is approximately 25.9 kDa. The enzyme is synthesized as an inactive zymogen, proelastase,[10] which is converted to the active form by limited proteolysis at the N-terminal by trypsin.
Other Notes
One unit will hydrolyze 1.0 μmole of N-succinyl-L-Ala-Ala-Ala-p-nitroanilide per min, pH 8.0 at 25 °C.
Packaging
Package size based on protein content
Physical form
Contains sodium carbonate.
Preparation Note
A further purification of Type III, E 0127, by affinity chromatography to reduce trypsin activity
related product
Product No.
Description
Pricing
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Pancreatic elastases
Handbook of Proteolytic Enzymes, 2639-2645 (2013)
Cloning and overexpression of extracellular elastase from Pseudomonas aeruginosa
Raftari M, et al.
European journal of inflammation, 11(1), 55-60 (2013)
Elastase Digests: New Ammunition for Shotgun Membrane xn-ProteomicsS-ig5f
Rietschel B, et al.
Molecular and Cellular Proteomics, 8(5), 1029-1029 (2009)
Regulation of human aortic endothelial cell-derived mesenchymal growth factors by allogeneic lymphocytes in vitro. A potential mechanism for cardiac allograft vasculopathy.
Wagner CR, et al.
The Journal of Clinical Investigation, 1269-1277 (1993)
N Rugani et al.
Biochimica et biophysica acta, 1247(2), 185-194 (1995-03-15)
Porcine colipase, the protein cofactor of pancreatic lipase, was isolated from pancreas freshly collected on animals and from a side fraction from the production of insulin (Novo Nordisk A/S). Samples of purified colipase were analyzed for homogeneity by polyacrylamide gel
Protocols
Test enzymatyczny elastazy (EC 3.4.21.36), ciągłe spektrofotometryczne oznaczanie szybkości.
Enzymatic Assay of Elastase (EC 3.4.21.36), a continuous spectrophotometric rate determination.
-
How does this product (elastase type IV) needs to be dissolved?
1 answer-
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service