Merck
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E4142

Sigma-Aldrich

ExtrAvidin®−Cy3

buffered aqueous solution

MDL number:
NACRES:
NA.46

conjugate

CY3 conjugate

Quality Level

form

buffered aqueous solution

extent of labeling

3-9 mol Cy3 per mol protein

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

shipped in

wet ice

storage temp.

2-8°C

Application

ExtrAvidin®-Cy3 is suitable for the following applications:
  • Immunohistochemistry (formalin-fixed, paraffin-embedded sections) at a dilution of 1:100
  • Immunocytochemistry.[1]
  • Used for labeling.[2]

Packaging

1 mL in glass bottle

Biochem/physiol Actions

Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs.[3] It consists of four high affinity binding sites for biotin. ExtrAvidin® is prepared from egg white avidin. It is a modified form of affinity purified avidin and binds biotin with high specific activity of avidin with the low background staining of streptavidin, a biotin binding protein produced by the bacteria Streptomyces avidinii.[4] ExtrAvidin® is covalently conjugated to Cy3 dye and is used for various techniques.

Physical form

Solution in 0.01 M phosphate buffered saline, containing 1% bovine serum albumin and 15 mM sodium azide

Preparation Note

Affinity purified protein

Legal Information

Cy3 is a trademark of Cytiva
ExtrAvidin is a registered trademark of Sigma-Aldrich Co. LLC

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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More Documents

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