This product has not been tested for use in cell culture. While Benzonase is used to reduce viscosity in protein solutions, there is no cell culture tested product available, currently. Note that glycerol may be inhibitory or cytotoxic at high concentrations.
E8263
Benzonase® Nuclease, ultrapure
≥250 units/μL, ≥99% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution, ultrapure grade
1 of 1 reviewers received a sample product or took part in a promotion
Synonym(s):
Endonuclease from Serratia marcescens
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| Size/SKU | Availability | Price |
|---|---|---|
5 kU | Please contact Customer Service for Availability | 835,00 PLN |
25 kU | Please contact Customer Service for Availability | 2.910,00 PLN |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
MDL number:
Assay:
≥99% (SDS-PAGE)
Biological source:
Serratia marcescens
Recombinant:
expressed in E. coli
Concentration:
≥250 units/μL
biological source
Serratia marcescens
Quality Segment
recombinant
expressed in E. coli
grade
ultrapure grade
assay
≥99% (SDS-PAGE)
form
buffered aqueous glycerol solution
mol wt
30 kDa
concentration
≥250 units/μL
input
general use sample(s)
application(s)
research use
shipped in
wet ice
storage temp.
−20°C
Application
The enzyme has been used for an assay to detect the amount of Tf1 RNA that is protected from degradation by the nuclease, Benzonase. The Tf1 element of Schizosaccharomyces pombe is a long terminal repeat-containing retrotransposon that encodes functional protease, reverse transcriptase, and integrase proteins. It has also been used in the fractionation of nuclear pellet and nuclear extract obtained from cell lysates.
Ultrapure benzonase nuclease, or Endonuclease from Serratia marcescens, has been used in a study to assess the effect of sodium dodecylbenzenesulfonate and antifoaming agents on the endonuclease activity of Serratia marcescens. Endonuclease from Serratia marcescens has also been used in a study to investigate the structure of chromatin subunits.
Used for the removal of nucleic acid from protein samples.
Biochem/physiol Actions
Benzonase® is a genetically engineered endonuclease from Serratia marcescens. The protein is a dimer of 30 kDa subunits with two essential disulfide bonds. This endonuclease attacks and degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) and is effective over a wide range of operating conditions. It completely digests nucleic acids to 5′- monophosphate terminated oligonucleotides 3 to 5 bases in length. This is ideal for the removal of nucleic acids from recombinant proteins. It can also be used for applications where complete digestion of nucleic acids is desirable. It also reduces viscosity in protein extracts and prevents cell clumping. Pre-treatment of a protein sample with this enzyme improves its resolution on 2D gel electrophoresis by eliminating any bound nucleic acids. The optimum pH for enzyme activity is found to be 8.0-9.2.
Digests native or heat-denatured DNA and RNA.
Physical form
Solution in 50% glycerol containing 20 mM Tris HCl, pH 8.0, 2 mM MgCl2, and 20 mM NaCl.
Other Notes
One unit will digest sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a ΔA260 of 1.0 in 30 min at pH 8.0 at 37 °C (reaction volume 2.625 ml).
Legal Information
Benzonase® Nuclease is supplied by Merck KGaA, Darmstadt, Germany and/or its affiliates.
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
1 of 1
This Item | |||
|---|---|---|---|
| description ≥250 units/μL, ≥99% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution, ultrapure grade | description Effective viscosity reduction and removal of nucleic acids from protein solutions | description - | description Effective Viscosity Reduction and Removal of Nucleic Acids from protein solutions |
| assay ≥99% (SDS-PAGE) | assay >99% (SDS-PAGE) | assay >90% (SDS-PAGE) | assay >99% (SDS-PAGE) |
| biological source Serratia marcescens | biological source Serratia marcescens | biological source Serratia marcescens | biological source Serratia marcescens |
| grade ultrapure grade | grade - | grade - | grade - |
| recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in E. coli |
| form buffered aqueous glycerol solution | form buffered aqueous glycerol solution | form buffered aqueous glycerol solution | form buffered aqueous glycerol solution |
| application(s) research use | application(s) research use | application(s) research use | application(s) research use |
Storage Class
10 - Combustible liquids
WGK
WGK 1
PPE (personal protective equipment)
Eyeshields, Gloves
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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Can this benzonase be used for cell culture? I have a protocol to use benzonase to reduce the clumps while resuspending the cells derived from tissue.
1 answer-
Helpful?
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Hello, I purchased it recently while the mailroom didn't notify me immediately when it arrived, so it stayed in 4 degree instead or -20 for around two days. Could you please provide me some information if it will highly affect the efficiency of the enzyme
1 answer-
Benzonase Nuclease is extremely stable. Even with extended incubations at 37 °C, Benzonase® Nuclease maintained > 90% activity for several months.
Helpful?
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Does this benzonase contain any epitope tag?
1 answer-
Product E8263 does not have any epitope tag.
Helpful?
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