F1056

Fibronectin human plasma

lyophilized powder, BioReagent, suitable for cell culture, ≥95% (SDS-PAGE)

• Synonym(s): Fibronectin
• EC Number: 289-149-2
• MDL number: MFCD00131062
• NACRES: NA.75

Properties

• Quality Level: 200
• biological source: human plasma
• sterility: sterile
• product line: BioReagent
• assay: ≥95% (SDS-PAGE)
• form: lyophilized powder
• packaging: pkg of 1 mg; pkg of 2 mg; pkg of 5 mg
• technique(s): cell culture | mammalian: suitable
• surface coverage: 1‑5 μg/cm²
• solubility: H₂O: 1 mg/mL at 37 °C
• UniProt accession no.: P02751
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... FN1(2335)

Description

Caution

The reconstituted solution should be stored in working aliquots at -20°C or lower. Vortexing, excessive agitation, or additoinal freezing and thawing of reconstituted fibronectin are not recommended.

Preparation Note

This product is lyophilized from 0.05 M Tris buffered saline at pH 7.5. It is soluble at 1 mg/mL in water, and should be allowed to dissolve for at least 30 minutes at 37°C. A small amount of undissolved material may remain, but it will not affect performance. The protein homogeneity is evaluated by immunoelectrophoresis, is endotoxin tested, and the source material was tested negative for HIV, hepatitis B and hepatitis C.

In coating culture surfaces, fibronectin should be diluted in sterile balanced sterile salt solution and coated with minimal volume. Surface should then be air dried for 45 minutes at room temp and can be stored for 2-4 weeks at 2-8°C.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves