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F2928

Sigma-Aldrich

Monoclonal Anti-Fas Ligand antibody produced in rat

clone 101624, purified immunoglobulin, lyophilized powder

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Synonym(s):
Anti-CD95L, Anti-FasL
MDL number:
NACRES:
NA.41

biological source

rat

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

101624, monoclonal

form

lyophilized powder

species reactivity

mouse

technique(s)

neutralization: suitable
western blot: 1-2 μg/mL

isotype

IgG1

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

mouse ... Fasl(14103)

Related Categories

General description

Fas Ligand is a 40kDa type II membrane protein, which also known as FasL and CD95L, belongs to the tumor necrosis factor (TNF) family. In the new TNF superfamily nomenclature, FasL is referred as TNFSF6. It consists of TNFα, α and β chains of lymphotoxin (LT), CD40 ligand, and CD30 ligand. It also has four potential N-glycosylation sites which appear to be variably used.

Immunogen

purified recombinant mouse Fas Ligand, amino acids 132-279, expressed in NSO cells.

Application

The monoclonal Anti-Fas Ligand antibody is suitable for western blot analysis of rat thymus homogenates to detect the recombinant rFasL molecule. The antibody is also suitable for immunoblotting at a concentration of 1-2μg/ml and ELISA at a concentration of 0.5-1.0μg/ml.

Biochem/physiol Actions

FasL is predominantly expressed on activated T cells and NK cells, whereas Fas is expressed on various cell types. The gene expression is induced by the activation of mature T cells with phorbol myristic acetate (PMA) and ionomycin, concanavalin A (Con A) or anti-CD3. After cleaving of FasL, it can generate the soluble Fas ligand, a non-covalently linked homotrimer. Membrane-bound FasL and TNFα are primary activators of their receptors. Soluble FasL may inhibit the killing effect of membrane FasL. It plays an important role in modulating immune response by inducing cell apoptosis to maintain homeostasis, self-tolerance of lymphocytes, and immune privilege. It has chemoattractant characteristics for neutrophils which indicate proinflammatory function.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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