As indicated above on this PDP, the immunogen for this polyclonal antibody is ferritin isolated from normal human spleen. Sequence information can be found in GenBank at the following link:
https://www.uniprot.org/uniprotkb/P02794/entry#sequences
F5012
Anti-Ferritin, Human antibody produced in rabbit
whole antiserum, liquid
Synonym(s):
Anti-FHC, Anti-FTH, Anti-FTHL6, Anti-HFE5, Anti-PIG15, Anti-PLIF
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| Size/SKU | Availability | Price |
|---|---|---|
2 mL | Please contact Customer Service for Availability | 1.920,00 PLN |
About This Item
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ODD
Citations:
51
biological source
rabbit
Quality Segment
conjugate
unconjugated
antibody form
whole antiserum
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
15 mM sodium azide
species reactivity
human
technique(s)
Ouchterlony double diffusion: 1:16
UniProt accession no.
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... FTH1(2495)
General description
Ferritin, an iron-storage protein is usually present in the liver and spleen in mammals. Iron and ferritin are distributed relatively same in the eye. Ferritin is made up of heavy and light chains.
Immunogen
Ferritin isolated from normal human spleen
Application
- Anti-Ferritin, Human antibody produced in rabbit has been used in:
- immunohistochemistry
- western blotting
- immunocytochemical techniques
Anti-Ferritin, Human antibody is suitable for use in Ouchterlony double diffusion (1:16 using human spleen ferritin), and immunoelectrophoresis. The antibody may also be used in western blot (1:1000 using frozen liver homogenates) and immunoprecipitation (using 300 μg of cell lysate incubated with 6 mul of anti-ferritin for 1h at 4 ° C).
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Biochem/physiol Actions
Ferritin is an iron-storage protein that regulates iron-homeostasis in cells. While mitochondrial ferritin is a homopolymer, the cytosolic form consists of heteropolymeric L- and H-chains. Genetic mutations in ferritin L-chains have been associated with neurodegenerative disorders, whereas altered levels of ferritin have been linked to hemophagocytic lymphohistiocytosis and adult-onset Still′s disease . In immunoelectrophoresis, anti-ferritin, human antibody is specific for ferritin when used against human spleen extract and purified ferritin. No reactivity is observed against normal human serum.
Preparation Note
The antiserum has been treated to remove lipoproteins.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| description whole antiserum, liquid | description purified immunoglobulin, buffered aqueous solution | description affinity isolated antibody | description ~1.0 mg/mL, clone FTH24, purified immunoglobulin |
| antibody form whole antiserum | antibody form purified immunoglobulin | antibody form affinity isolated antibody | antibody form purified immunoglobulin |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| biological source rabbit | biological source rabbit | biological source rabbit | biological source mouse |
| Quality Level 200 | Quality Level 100 | Quality Level 100 | Quality Level 200 |
| technique(s) Ouchterlony double diffusion: 1:16 | technique(s) western blot: 1 μg/mL | technique(s) immunohistochemistry: suitable, immunoblotting: suitable | technique(s) immunoblotting: 0.5-1.0 μg/mL using total cell extracts of HEK-293T cells overexpressing FTH1 (expected: ~26kDa)., indirect immunofluorescence: 5-10 μg/mL using HeLa cells, flow cytometry: 10-20 μg/test using HeLa cells |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
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Storage Class
12 - Non Combustible Liquids
WGK
nwg
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