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F8799

Sigma-Aldrich

Fas/Fc Chimera from mouse

>97% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture

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Synonym(s):
Apo-1, CD95
MDL number:
NACRES:
NA.32

recombinant

expressed in baculovirus infected Sf21 cells

Quality Level

Assay

>97% (SDS-PAGE)

form

lyophilized powder

potency

0.03-0.1 μg per mL ED50 (in the presence of 20 ng/mL recombinant human Fas ligand for cytotoxicty assay of Jurkat cells)

mol wt

55 kDa (glycosylated form)

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

UniProt accession no.

storage temp.

−20°C

Gene Information

mouse ... Fas(14102)

Related Categories

General description

Fas is a key component of apoptosis, and is a cell surface receptor. This gene is localized to human chromosome 10q24.1, and is composed of nine exons and eight introns.

Application

Fas/Fc Chimera from mouse has been used as a blocking agent during FACS (flow cytometry).

Biochem/physiol Actions

Fas and its ligand play an essential role in apoptosis, by activating the cell death signaling pathway. Mutations in this gene are associated with evasion of apoptosis by cells, leading to tumorigenesis, and prevention of clearance of these cells by the immune system. A polymorphism in this gene results in the production of soluble Fas (sFas), which is implicated in the chronic autoimmune disorder called primary Sjögren′s syndrome (pSS).

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 50 μg bovine serum albumin per 1 μg of cytokine.

Analysis Note

Biological activity was measured by its ability to inhibit Fas-ligand induced apoptosis of Jurkat cells.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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