F8799

Fas/Fc Chimera from mouse

>97% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture

• Synonym(s): Apo-1, CD95
• MDL number: MFCD01867037
• NACRES: NA.32

Properties

• recombinant: expressed in baculovirus infected Sf21 cells
• Quality Level: 200
• Assay: >97% (SDS-PAGE)
• form: lyophilized powder
• potency: 0.03-0.1 μg per mL ED₅₀ (in the presence of 20 ng/mL recombinant human Fas ligand for cytotoxicty assay of Jurkat cells)
• mol wt: 55 kDa (glycosylated form)
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• UniProt accession no.: P25446
• storage temp.: −20°C
• Gene Information: mouse ... Fas(14102)

Description

General description

Fas is a key component of apoptosis, and is a cell surface receptor. This gene is localized to human chromosome 10q24.1, and is composed of nine exons and eight introns.

Application

Fas/Fc Chimera from mouse has been used as a blocking agent during FACS (flow cytometry).

Biochem/physiol Actions

Fas and its ligand play an essential role in apoptosis, by activating the cell death signaling pathway. Mutations in this gene are associated with evasion of apoptosis by cells, leading to tumorigenesis, and prevention of clearance of these cells by the immune system. A polymorphism in this gene results in the production of soluble Fas (sFas), which is implicated in the chronic autoimmune disorder called primary Sjögren′s syndrome (pSS).

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 50 μg bovine serum albumin per 1 μg of cytokine.

Analysis Note

Biological activity was measured by its ability to inhibit Fas-ligand induced apoptosis of Jurkat cells.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable