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Merck

G1924

L-Glutamate Oxidase from Streptomyces sp.

recombinant, expressed in E. coli

Synonym(s):

L-Glutamate:oxygen oxidoreductase (deaminating)

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G1924-1UN

PLN 588.00

G1924-5UN

PLN 1,970.00

PLN 588.00

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About This Item

CAS Number:
39346-34-4
MDL number:
MFCD00131459
UNSPSC Code:
12352204
NACRES:
NA.54

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recombinant

expressed in E. coli

Quality Level

200

form

solid

specific activity

≥5 units/mg protein

mol wt

120 kDa

storage temp.

−20°C

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1 of 4

This Item
G1270G5921C8649
L-Glutamate Oxidase from Streptomyces sp. recombinant, expressed in E. coli

Sigma-Aldrich

G1924

L-Glutamate Oxidase from Streptomyces sp.

L-Glutamine Synthetase from Escherichia coli lyophilized powder, 400-2,000 units/mg protein

Sigma-Aldrich

G1270

L-Glutamine Synthetase from Escherichia coli

L-Glutamate Oxidase from Streptomyces sp. recombinant, expressed in E. coli, lyophilized powder, ≥5.0 units/mg solid

Sigma-Aldrich

G5921

L-Glutamate Oxidase from Streptomyces sp.

Cholesterol Oxidase from Streptomyces sp. lyophilized powder, ≥20 units/mg protein

Sigma-Aldrich

C8649

Cholesterol Oxidase from Streptomyces sp.

specific activity

≥5 units/mg protein

specific activity

400-2,000 units/mg protein

specific activity

≥5.0 units/mg solid

specific activity

≥20 units/mg protein

recombinant

expressed in E. coli

recombinant

-

recombinant

expressed in E. coli

recombinant

-

form

solid

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

mol wt

120 kDa

mol wt

-

mol wt

-

mol wt

~34 kDa

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

L-Glutamate Oxidase (LGOX) from Streptomyces sp is secreted extracellularly in the wheat bran pre cultivation medium. It corresponds to a molecular weight of 120 kDa with monomer subunit of 61 kDa.[1] The structure of LGOX comprises of hexamer and has flavin adenine dinucleotide prosthetic group.[2]
This product has a 6xHistidine tag with a thrombin cleavage site.

Application

L-Glutamate Oxidase from Streptomyces sp may be used:
  • as a reference enzyme in hydrogelpoly(carbamoylsulphonate) based immobilization for biosensor development[3]
  • as a component of pencil graphite electrode for biosensing L-glutamate[4]

Biochem/physiol Actions

L-Glutamate Oxidase (LGOX) catalyzes the oxidative deamination of L-glutamate to α-ketoglutarate, ammonia and hydrogen peroxide.[1] LGOX is industrially produced and is exploited majorly as a biosensor for L-glutamate detection and in the L-glutamate kits.[2]

Other Notes

One unit will form 1.0 micromole of alpha-ketoglutaric acid from L-glutamic acid per minute at pH 7.4 at 30 deg C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000427395
0000427395
0000356452
0000356451
0000356452

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L-Glutamate biosensor based on l-glutamate oxidase immobilized onto ZnO nanorods/polypyrrole modified pencil graphite electrode
Batra B, et al.
Biochemical Engineering Journal, 105, 428-436 (2016)
Isolation, purification, and characterization of L-glutamate oxidase from Streptomyces sp. 18G
Wachiratianchai S, et al.
Electronic journal of Biotechnology, 7(3), 09-10 (2004)
Comparative study of hydrogel-immobilized L-glutamate oxidases for a novel thick-film biosensor and its application in food samples
Kwong AWK, et al.
Biotechnology Letters, 22(4), 267-272 (2000)
Jiro Arima et al.
The FEBS journal, 276(14), 3894-3903 (2009-06-18)
L-Glutamate oxidase (LGOX) from Streptomyces sp. X-119-6, which catalyzes the oxidative deamination of L-glutamate, has attracted increasing attention as a component of amperometric L-glutamate sensors used in the food industry and clinical biochemistry. The precursor of LGOX, which has a
Andrea C Kneuttinger et al.
Cell chemical biology, 26(11), 1501-1514 (2019-09-10)
Imidazole glycerol phosphate synthase (ImGPS) is an allosteric bienzyme complex in which substrate binding to the synthase subunit HisF stimulates the glutaminase subunit HisH. To control this stimulation with light, we have incorporated the photo-responsive unnatural amino acids phenylalanine-4'-azobenzene (AzoF)

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