Merck
All Photos(1)

GE28-9909-44

Sigma-Aldrich

Superdex® 200 Increase 10/300 GL

Cytiva 28-9909-44, column L × I.D. 30 cm × 10 mm, 8.6 μm particle size

NACRES:
SB.52

material

glass column
polyethylene filter

product line

Superdex®

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

mol wt

10000-600000 Da (globular proteins)

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 28-9909-44

parameter

<1.8 mL/min flow rate
<1.80 mL/min flow rate
3.0 Mpa (580 psi)

bed volume

~24 mL

column L × I.D.

30 cm × 10 mm

sample volume

25-500 μL

matrix

agarose-dextran composite
polymeric

particle size

8.6 μm

cleaning

1-14

working range

3-12

separation technique

size exclusion (SEC)

General description

Superdex Increase 200 is a column for high resolution gel filtration in small scale (mg) preparative purification and also for characterization and analysis of proteins with molecular weights between 10,000 and 600,000, such as aggregate analysis of antibodies, size-homegeneity analysis of membrane proteins, or protein-protein interactions. Pre-packed with small rigid agarose beads for high flow rates and pH stability.

Suitability

For both preparative and analytical purposes
Gel filtration for small-scale (25-500 mL) preparative purification.
Long term use: all commonly used aqueous buffers, up to 8 M urea, ionic and non-ionic detergents, up to 6M guanidine hydrochloride

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Superdex is a registered trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Storage Class Code

3 - Flammable liquids

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service