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M2133

4-Methylumbelliferyl N-acetyl-β-D-glucosaminide

β-N-acetylhexosaminidase substrate, fluorogenic, ≥98% (TLC), powder

Synonym(s):

4-Methylumbelliferyl-2-acetamido-2-deoxy-β-D-gluco­pyrano­side

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Size/SKUAvailabilityPrice
25 mg
Please contact Customer Service for Availability
286,00 PLN
100 mg
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963,00 PLN
250 mg
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1.590,00 PLN
1 g
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4.230,00 PLN

About This Item

Empirical Formula (Hill Notation):
C18H21NO8
CAS Number:
Molecular Weight:
379.36
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.32
EC Number:
253-333-0

286,00 PLN


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Product Name

4-Methylumbelliferyl N-acetyl-β-D-glucosaminide, β-N-acetylhexosaminidase substrate

Quality Segment

assay

≥98% (TLC)

form

powder

solubility

DMF: 20 mg/mL, clear, colorless to faintly yellow

storage temp.

−20°C

SMILES string

CC(=O)N[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1Oc2ccc3C(C)=CC(=O)Oc3c2

InChI

1S/C18H21NO8/c1-8-5-14(22)26-12-6-10(3-4-11(8)12)25-18-15(19-9(2)21)17(24)16(23)13(7-20)27-18/h3-6,13,15-18,20,23-24H,7H2,1-2H3,(H,19,21)/t13-,15-,16-,17-,18?/m1/s1

InChI key

QCTHLCFVVACBSA-XWEABGILSA-N

General description

4-Methylumbelliferyl N-acetyl-β-D-glucosaminide (4-MUF-NAG) contains chitin monomer, N-acetyl-D-glucosamine (NAG) and a 4-methylumbelliferone (7-hydroxy-4-methylcoumarin) (4-MUF) moiety.
Fluorogenic substrate for β-N-acetylhexosaminidase

Application

4-Methylumbelliferyl N-acetyl-β-D-glucosaminide has been used as a substrate for assaying
  • β-N-acetylglucosaminidase (NAGase) from soil samples
  • chitinase from Fusarium oxysporum using fluorimetric assay
  • β-N-acetylhexosaminidases from Arabidopsis thaliana roots and leaves samples

Biochem/physiol Actions

4-Methylumbelliferyl N-acetyl-β-D-glucosaminide (4-MUF-NAG) is a synthetic fluorogenic substrate for N-acetyl-β-D-glucosaminidase (NAGase). The enzyme functionality is assessed by its ability to hydrolyze the N-acetyl-D-glucosamine (NAG) residue in 4-MUF-NAG. 4-MUF-NAG is also a substrate for Chitinase and N-acetylhexosaminidase.

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This Item
47450245442869585
description

β-N-acetylhexosaminidase substrate

description

Ultrasensitive, fluorogenic substrate for assays of N-acetyl-β-D-glucosaminidase.

description

Fluorogenic substrate for isoenzyme A of N-acetyl-β-D-glucosaminidase (hexosaminidase A). Can be used for the prenatal diagnosis of Tay-Sachs disease and for the classification of GM2 gangliosidosis genotypes.

description

suitable for fluorescence, ≥99.0% (TLC)

assay

≥98% (TLC)

assay

≥98% (TLC)

assay

≥98% (HPLC)

assay

≥99.0% (TLC)

solubility

DMF: 20 mg/mL, clear, colorless to faintly yellow

solubility

methanol: soluble, water: soluble

solubility

water: 20 mg/mL

solubility

DMF: 20 mg/mL, clear, colorless

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

100

form

powder

form

crystalline solid

form

crystalline solid

form

powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C


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Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)



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