MTOX1024

HepaRG^™ Cells PERK (-/-)

human female liver (hepatocarcinoma and hepatitis C tumor)

• UNSPSC Code: 12352207
• NACRES: NA.81
• Biological source: human female liver (hepatocarcinoma and hepatitis C tumor)

Properties

• Product Name: HepaRG^™ Cells PERK (-/-), one vial
• biological source: human female liver (hepatocarcinoma and hepatitis C tumor)
• Quality Segment: 200
• form: liquid
• OMIM accession no.: 604032
• storage temp.: −196°C
• Gene Information: human ... EIF2AK3(9451)

Description

General description

HepaRG is a human hepatoma cell line. The cells possess a pseudodiploid karyotype and have been characterized as an oval ductular bipotent hepatic cell line as they have the ability to differentiate into both biliary and hepatocyte lineages in the presence of DMSO. HepaRG PERK knockout cells express the major xenobiotic sensors (PXR, CAR and AhR), drug transporters, phase I and II drug metabolizing enzymes as well as key hepatic transcription factors involved in stress response pathways.

Application

See technical bulletin for detailed protocols

Features and Benefits

HepaRG cells are the most metabolically active human hepatocyte cell line developed to date. These the cells are suitable for a wide variety of studies for drug metabolism, CYP induction, metabolism-mediated toxicity, transporter, and hepatotoxicity. - Sigma′s HepaRG PERK Knockout (KO) allows investigations of Wolcott-Rallison syndrome and endoplasmic reticulum stress response involving PERK in the liver.

Analysis Note

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.

Legal Information

Exhibit 1: ADME/Tox cell lines license
Exhibit 2: HepaRG limited use license

HepaRG is a trademark of BioPredic International company

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable