N1408

Nerve Growth Factor β Human

>97% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture

• Synonym(s): HSAN5, NGF-β human, NGFB, hNGFβ
• UNSPSC Code: 12352202
• NACRES: NA.77
• MDL number: MFCD00164982

Properties

• Product Name: NGF-β human, from human, recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture
• biological source: human
• recombinant: expressed in NSO cells
• assay: >97% (SDS-PAGE)
• form: lyophilized powder
• potency: 0.15-3.0 ng/mL ED₅₀/EC₅₀
• quality: endotoxin tested
• packaging: pkg of 5X0.1 mg; pkg of 0.1 mg
• technique(s): cell culture | mammalian: suitable
• impurities: <1 EU/μg
• solubility: water: soluble 0.1 g/L at 20 °C
• UniProt accession no.: P01138
• storage temp.: −20°C
• Quality Level: 200
• Gene Information: human ... NGF(4803)

Description

Analysis Note

The biological activity is measured in a cell proliferation assay using a factor-dependent human erythroleukemic cell line, TF-1.

Application

NGF-β human has been used:

• in protein pin arrays[1]
• to induce U87-MG (glioblastoma cell line) cell proliferation[2]
• in drug release kinetics[3]

Biochem/physiol Actions

Nerve growth factor β (NGFB) acts as a key mediator in neuronal and non-neuronal development, differentiation,[4][5] and survival.[5] Polymorphism of NGFB gene leads to hereditary sensory and autonomic neuropathies. Charcot arthropathy and thermal insensitivity are few of the related symptoms.[4] NGFβ is known to promote cancer cell proliferation and metastasis, also mediates angiogenesis.[6][7] It facilitates the development of various cancer including, oral,[7] breast, lungs, pancreatic and prostate cancers.[6][7] Elevated expression of NGFβ contributes to progression of human cholangiocarcinoma.[7] In animal models, NGFβ is observed to prevent cholinergic neuron loss and enhances memory.[8] NGF-β promotes the survival and cholinergic phenotype of basal forebrain cholinergic neurons and stimulates neurite outgrowth of embryonic trigeminal motor neurons in vitro.

General description

The nerve growth factor β (NGFB) gene is mapped to human chromosome 1p13.2.[4]

Physical form

Lyophilized from 0.2% acetic acid containing 5 mg bovine serum albumin.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)