RAB0543

Human IL18 / Interleukin-18 ELISA Kit

for serum, plasma, cell culture supernatants and urine

• Synonym(s): IFN-gamma-inducing factor, IL-18, Interleukin-18
• NACRES: NA.32
• UNSPSC Code: 41116158

Properties

• Product Name: Human IL18 / Interleukin-18 ELISA Kit, for serum, plasma, cell culture supernatants and urine
• species reactivity: human
• packaging: kit of 96 wells (12 strips x 8 wells)
• technique(s): ELISA: suitable
• input: sample type cell culture supernatant(s); sample type urine; sample type plasma; sample type serum
• assay range: inter-assay cv: <10%; intra-assay cv: <12%; sensitivity: 0.5 pg/mL
• detection method: colorimetric
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... IL18(3606)

Description

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Human IL18 / Interleukin-18 ELISA Kit has been used to evaluate the macrophage cytokines production in response to trans-stilbene and terphenyl compounds.[1] It has also been used to analyze and measure IL-18 levels in the urine of chronic kidney disease patients.[2]

General description

The antibody pair provided in this kit detects Human IL-18 in serum, plasma, cell culture supernatants, and urine. Interleukin-18 (IL-18) is a proinflammatory cytokine,[3] expressed in most of the mammalian tissues including adipose, liver and skeletal muscle.[4] It is generated as pro-IL-18 which undergoes cleavage to form a mature IL-18 possessing the signal peptide.[4] IL-18 induces Th1 (T helper cell 1) lymphocyte action mediated by interferon-γ.[5] It controls the metabolism of lipids and glucose.[4] Inflammasome activation is responsible for IL-18 release, which is a significant event in response to viral infection.[3] Increased level of IL-18 is observed in kidney and liver disease, diabetes, atherosclerosis,[3] autoimmune disease such as rheumatoid arthritis and asthma.[5] It is also associated with the development of renal dysfunction.[3]

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Safety Information

• pictograms: GHS05
• signalword: Warning
• hcodes: H290
• pcodes: P234 - P390
• Hazard Classifications: Met. Corr. 1
• Storage Class: 8A - Combustible corrosive hazardous materials
• flash_point_f: Not applicable
• flash_point_c: Not applicable