S0902

SSC Buffer

for Northern and Southern blotting, powder blend

• Synonym(s): Saline-sodium citrate buffer
• NACRES: NA.25

Properties

• Quality Level: 200
• form: powder blend
• pH: 6.8-7.2 (20-25 °C, 263 mg/L in water)
• solubility: water: 263 mg/mL, clear, colorless
• foreign activity: DNAse, none detected; Endonuclease, none detected; Exonuclease, none detected; NICKase, none detected; RNAse, none detected
• storage temp.: room temp

Description

General description

SSC Buffer 20x Powder is a standard reagent in Southern and Northern blotting procedures. This concentrated powder should be reconstituted in molecular biology grade water to the indicated final volume before use (final solution is a 20x). The 20x concentrate can be used straight or diluted to make SSC working solutions with concentrations as low as 0.5x.

Application

Saline-sodium citrate (SSC) Buffer has been used as a washing buffer in microarray technique.[1] It has also been used as a constituent of fluorescence in situ hybridization (FISH) buffer.[2]

Suitable for:

• Preparing nucleic acids for hybridization
• Hybridization buffer for Northern and Southern blots
• Washing buffer for Northern and Southern blots

Features and Benefits

• Multi-purpose use in hybridizations
• Easily reconstituted and diluted to any concentration needed

Components

Final SSC Buffer 20x Concentrate contains 0.3 M sodium citrate in 3M NaCl.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable