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SHPH01

Sigma-Aldrich

MISSION® LentiPlex® Human Pooled shRNA Library

For Rapid, Convenient Genome-wide shRNA Screens

NACRES:
NA.51

Quality Level

product line

MISSION®

concentration

≥5x108 VP/ml (via p24 assay)

application(s)

capture ELISA: 5 × 108 TU/mL using p24

shipped in

dry ice

storage temp.

−70°C

General description

MISSION LentiPlex Pooled Libraries bring the power of the MISSION TRC shRNA collection together with Sigma¢s lentiviral manufacturing expertise to enable genome-wide RNAi screens at your bench-top. The LentiPlex pooled system provides enhanced delivery and long-term gene silencing in non-dividing and primary cell lines allowing unlimited discovery potential. The convenient format now makes rapid whole-genome RNAi screens accessible to any researcher with minimal reagent, time or capital equipment investment.

The MISSION LentiPlex Human shRNA Pooled Library is a genome-wide lentiviral pool produced using a proprietary process. The library consists of over 75,000 shRNA constructs from the TRC collection targeting 15,000+ human genes. Each library is tested for shRNA representation before product release to ensure robust library coverage. The library is provided in ready-to-use lentiviral format at titers of at least 5 x 108 TU/ml via p24 assay and is pre-divided into ten subpools of approximately 8,000 shRNA constructs each. Amplification and sequencing primers are also provided for downstream target identification.

The library is pre-divided into ten subpools of approximately 8,000 shRNA constructs each. Each library is enough material for six screens at 100X representation.

Please visit sigma.com/lentiplex to learn more and see example data and uses.

Application

MISSION® LentiPlex® Human Pooled shRNA Library has been used to transfect human colon adenocarcinoma cell line HT29 for shRNA screening and bioinformatics analysis.

Other Notes

Deconvolution of shRNA Pools
Sigma′s deconvolution service lets you easily identify the genes that impact your pooled shRNA screen.

  • Next-generation sequencing of clonesgives a precise number of individual clone occurrence within a pooled shRNA sample
  • Comprehensive, reproducible results from pooled shRNA screens
  • Statistically robust and information-rich data

Contact your local Sigma sales representative for more information or submit an inquiry to MISSION RNAi

Legal Information

LentiPlex is a registered trademark of Sigma-Aldrich Co. LLC
MISSION is a registered trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Rong Geng et al.
Chinese journal of cancer, 36(1), 63-63 (2017-08-02)
The mitogen-activated extracellular signal-regulated kinase 1/2 (MEK1/2) inhibitor trametinib has shown promising therapeutic effects on melanoma, but its efficacy on colorectal cancer (CRC) is limited. Synthetic lethality arises with a combination of two or more separate gene mutations that causes...
Ziyang Wang et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 7(17), 2000593-2000593 (2020-10-01)
Colorectal cancer (CRC) has become a predominant cancer worldwide. To understand the process of carcinogenesis, a short hairpin RNA library screening is employed to search for candidate genes that promote proliferation in the CRC cell line HT29. The candidate genes...
Xue-Cen Wang et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 25(14), 4567-4579 (2019-04-14)
Neoadjuvant chemoradiotherapy (neoCRT) is a standard treatment for locally advanced rectal cancer (LARC); however, resistance to chemoradiotherapy is one of the main obstacles to improving treatment outcomes. The goal of this study was to identify factors involved in the radioresistance...
Davide Zecchin et al.
EMBO molecular medicine, 12(8), e11987-e11987 (2020-07-17)
Triple-negative breast cancer (TNBC) has poorer prognosis compared to other types of breast cancers due to the lack of effective therapies and markers for patient stratification. Loss of PTEN tumor suppressor gene expression is a frequent event in TNBC, resulting...
Christine Karlsson et al.
Methods in molecular biology (Clifton, N.J.), 650, 29-43 (2010-08-06)
Identifying the genes and pathways that regulate self-renewal and differentiation in somatic stem cells is a central goal in stem cell and cancer biology. Here, we describe a method for RNAi-based screens in primary human hematopoietic stem and progenitor cells....

Articles

Pooled Lentiviral shRNA Screens to Identify Essential Cancer Pathways

Presenting an article on Pooled Lentiviral shRNA Screens to Identify Essential Cancer Pathways

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