After reconstituting the protein in water, it remains active and stable at 2-8°C for up to a week. However, for long-term stabilization at -20 to -80°C, further dilution with a buffer containing a carrier is necessary to prevent activity loss. It is recommended to use 5% trehalose or 0.1% BSA in water or PBS for extended storage. Working aliquots should have a minimum volume of 10 uL and a concentration above 1 ug/ml for optimal stability.
SRP3027
EGF human
Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonym(s):
Epidermal Growth Factor, URG, Urogastrone
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Select a Size
500 μG
PLN 2,340.00
About This Item
NACRES:
NA.32
UNSPSC Code:
12352202
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Product Name
EGF human, Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
biological source
human
recombinant
expressed in E. coli
assay
≥98% (HPLC)
≥98% (SDS-PAGE)
form
lyophilized
potency
≤0.2 ng/mL
mol wt
6.2 kDa
packaging
pkg of 500 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
color
white to off-white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... EGF(1950)
1 of 4
This Item | E9644 | SRP3182 | SRP3196 |
|---|---|---|---|
| assay ≥98% (HPLC), ≥98% (SDS-PAGE) | assay ≥97% (SDS-PAGE) | assay ≥98% (HPLC), ≥98% (SDS-PAGE) | assay ≥98% (HPLC), ≥98% (SDS-PAGE) |
| biological source human | biological source human | biological source human | biological source mouse |
| recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in E. coli |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| form lyophilized | form lyophilized powder | form lyophilized | form lyophilized |
| packaging pkg of 500 μg | packaging pkg of 5X0.2 mg, pkg of 0.2 mg, pkg of 0.5 mg | packaging pkg of 10 μg | packaging pkg of 500 μg |
Application
EGF (epidermal growth factor) human has been used in the culture medium for the maintenance of MCF10A cells (human mammary epithelial cells).[1]
Biochem/physiol Actions
EGF (epidermal growth factor) is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells.[2] Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing.[3][4] EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor.[5] This receptor also binds with TGF (transforming growth factor)-α and VGF (vaccinia virus growth factor). Mutation in the EGF gene might be associated with risk of non- small cell lung adenocarcinoma.[6] It is also involved in anaplastic thyroid cancer and renal hypomagnesemia. It controls cell differentiation and neurotrophic processes.[7][8]
General description
The gene EGF (epidermal growth factor) is mapped to human chromosome 4q25-27.[6] The EGF gene encodes for a large type I membrane–anchored precursor protein which is cleaved into pro-EGF for generation of peptide hormone EGF. The mRNA is expressed in kidney, salivary gland, cerebrum and prostate.[1] Recombinant human EGF is a 6.2kDa globular protein containing 53 amino acid residues including 3 intramolecular disulfide-bonds.
Other Notes
NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR
Physical form
Lyophilized with no additives.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Basal p21 controls population heterogeneity in cycling and quiescent cell cycle states.
Overton KW
Proceedings of the National Academy of Sciences of the USA, 111, E4386-E4393 (2014)
Neurotrophic actions of a novel molluscan epidermal growth factor.
Hermann PM
The Journal of Neuroscience, 20, 6355-6364 (2000)
Transforming growth factor alpha and epidermal growth factor in protection and healing of gastric mucosal injury.
Konturek SJ
Scandinavian Journal of Gastroenterology, 27, 649-655 (1992)
Clinical Implication of EGF A61G Polymorphism in the Risk of Non Small Cell Lung Adenocarcinoma Patients: A Case Control Study.
Masroor M
Asian Pacific Journal of Cancer Prevention, 16, 7529-7534 (2015)
The miR-200 family regulates the epithelial-mesenchymal transition induced by EGF/EGFR in anaplastic thyroid cancer cells.
Zhang Z
International Journal of Molecular Medicine, 30, 856-862 (2012)
Articles
Discover answers to your frequently asked questions about animal component-free, xenobiotic-free, and chemically defined media and reagents for cell culture.
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Is it necessary to dilute item no: SRP3027-500UG in a buffer with a stabilizer for extended storage as mentioned in the reconstitution portion of the specification. If so, would D-(+)-Trehalose dihydrate (cat#T0167) be appropriate? Also, specify the buffer considering that water is indicated as an appropriate diluent in the specifications.
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