• T8573
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Monoclonal Anti-Topoisomerase I antibody produced in mouse

clone Mab1, purified immunoglobulin, buffered aqueous solution

MDL number:

biological source


Quality Level



antibody form

purified immunoglobulin

antibody product type

primary antibodies


Mab1, monoclonal


buffered aqueous solution

mol wt

antigen ~100 kDa

species reactivity



microarray: suitable
western blot: 1-2 μg/mL using A231 human kidney cells



UniProt accession no.

shipped in

dry ice

storage temp.


Gene Information

human ... TOP1(7150)

General description

Monoclonal Anti-Topoisomerase I (mouse IgG1 isotype) is derived from the Mab1 hybridoma produced by the fusion of SP2/0 mouse myeloma cells and splenocytes from BALB/c mice immunized with human purified topoisomerase I. The protein family of DNA topoisomerases is divided into two types (type I and II). The type I enzymes include eukaryotic and bacterial topoisomerase I and III. Human DNA Topoisomerase I belong to the subtype IB enzymes. The protein has four distinct domains. There are four nuclear localization signals in the N-terminal domain. A linker domain of 77 amino acids follows the core domain. The fourth domain, which is 53 amino acids long, contains the active site Tyr723. Topoisomerase I (TOP1) gene is located on human chromosome 20q12.


purified human topoisomerase I


Monoclonal Anti-Topoisomerase I antibody produced in mouse has been used in western blot analysis.


200 μL in glass bottle

Biochem/physiol Actions

DNA topoisomerases are enzymes that control the amount of supercoiling in DNA. Without topoisomerases, DNA cannot replicate normally. Type I enzymes of DNA topoisomerases introduce transient single strand breaks in DNA. Recently it was reported that DNA topoisomerase I is involved in modulation of RNA and is present in retroviral particles, like human immunodeficiency virus (HIV1). Furthermore, the enzyme enhances HIV-1 cDNA production in reverse transcription assays in vitro. Topoisomerase I (TOP1) serves as swivel at the time of replication, transcription and recombination, which helps in releasing over winding of duplex DNA.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.


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Certificate of Analysis

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Certificate of Origin

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