Merck
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TP0300

Sigma-Aldrich

Total Protein Kit, Micro Lowry, Peterson′s Modification

NACRES:
NA.32

usage

sufficient for ~200 assays

Quality Level

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TP0200TP0100ACR5000PA
Millipore

Millipore

ACR5000PA

Protein A Reagent Kit

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

-

General description

This method utilizes sodium dodecylsulfate, included in the Lowry reagent, to facilitate the dissolution of relatively insoluble lipoproteins. An optional deoxycholate-TCA precipitation step permits removal of interfering substances such as Tris, sucrose, EDTA, salts, etc.

Application

Total Protein Kit, Micro Lowry, Peterson′s Modification has been used to measure protein content.

Features and Benefits

  • Complete kit for protein assays
  • Most widely cited protein assay
  • Less protein to protein variation than other assays
  • Contains reagents that can remove interfering substances

Principle

An optional deoxycholate-TCA precipitation step permits removal of interfering substances such as Tris, sucrose, EDTA, salts, etc. Absorbance at 500-800 nm (typically read at 750 nm) is used to determine protein concentration from a standard curve.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3


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Chiara Bernardini et al.
Journal of inflammation (London, England), 9(1), 47-47 (2012-11-28)
It is well known that nitric oxide (NO) is generated by a family of constitutively (nNOS and eNOS) or inducibly (iNOS) expressed enzymes and takes part in different aspects of the inflammatory response; nevertheless, its effective role in the pathogenesis
Aberrant glycosylation of alphavbeta3 integrin is associated with melanoma progression
Pochec E, et al.
Anticancer Research, 35(4), 2093-2103 (2015)
Alberto Elmi et al.
Animals : an open access journal from MDPI, 10(3) (2020-03-12)
The roe deer (Capreolus capreolus) represents a spontaneous model of testicular inactivation: During winter, bucks show a suspension of spermatogenesis that starts again in spring and peaks during the breeding season (July-August). The underlying mechanisms to the regulation of the
Chiara Bernardini et al.
BMC complementary and alternative medicine, 18(1), 194-194 (2018-06-27)
It is widely accepted the key role of endothelium in the onset of many chronic and acute vascular and cardiovascular diseases. In the last decade, traditional compounds utilized in "folk medicine" were considered with increasing interest to discover new bioactive
Dalia A Elgamal et al.
Molecular and cellular endocrinology, 503, 110700-110700 (2020-01-07)
Genetic and epigenetic factors contribute equally to the pathogenesis of type 1 diabetes mellitus. Sodium butyrate (NaB) has been reported to improve glucose homeostasis by modulation of the p38/ERK MAPK pathway. This work aims to evaluate the effect of NaB

Related Content

Protein Quantitation

Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.

Tools for Protein Quantitation

A wide variety of products for traditional protein quantitation techniques such as BCA and Bradford. Also, products for alternative assays such as Lowry, Micro Pyrogallol and FluoroProfile are also available for total protein determination.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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