67473-U

BIOshell^™ A400 Protein C18 (3.4 μm) HPLC Columns

L × I.D. 5 cm × 3 mm, HPLC Column

• UNSPSC Code: 41115700
• NACRES: SB.52

Properties

• Product Name: BIOshell^™ A400 Protein C18 Column, L × I.D. 5 cm × 3 mm, 3.4 μm particle size
• material: stainless steel hardware (column)
• agency: suitable for USP L1
• description: Shell Thickness (0.2 μm); Solid Core (3.0 μm)
• feature: endcapped
• analyte chemical class(es): proteins
• packaging: 1 ea of
• extent of labeling: 1% carbon loading
• parameter: 600 bar max. pressure; 90 °C max. temp.
• technique(s): HPLC: suitable; LC/MS: suitable; UHPLC-MS: suitable; UHPLC: suitable
• L × I.D.: 5 cm × 3 mm
• surface area: ~15 m²/g
• matrix: spherical silica particle platform; superficially porous particle
• matrix active group: C18 (octadecyl) phase
• particle size: 3.4 μm
• pore size: 3.4 μm particle size; 400 Å
• operating pH: 1-8
• separation technique: reversed phase
• Quality Level: 100
• product line: BIOshell^™
• manufacturer/tradename: BIOshell^™

Description

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

BIOshell is a trademark of Sigma-Aldrich Co. LLC

Application

UHPLC Analysis of Proteins on BIOshell^™ A160 Peptide C18 2.7 μm; BIOshell^™ A400 Protein C18, 3.4 μm; and BIOshell^™ IgG 1000 Å C18, 2.7 μm

General description

BIOshell^™ 400 Å Protein C18 columns contain 3.4 μm advanced Fused-Core^® particles with 400 Å pores that are specifically engineered to provide efficient separation of larger proteins. These columns offer maximum hydrophobicity for resolving tricky hydrophilic and PEGylated proteins. The 3.4 μm Fused-Core particles allow a shorter analysis time yet similar back pressure to fully porous 1.7 μm, 300 Å fully porous C18 particles. These columns can be operated long term at pressures up to 9000 psi (600 bar), temperatures up to 90 °C, and within the pH range of 1 to 9.