324713

Glu-C Protease

Selectively cleaves at glutamic acid residues, suitable for mass spectromety, from Staphylococcus aureus

• Synonim(y): Endoproteinase Glu-C, Excision Grade, Staphylococcus aureus, V8 Protease
• Numer CAS: 66676-43-5
• Numer WE: 3.4.21.19 (BRENDA, IUBMB)
• UNSPSC Code: 12352202
• NACRES: NA.54
• MDL number: MFCD01324998
• eCl@ss: 32160410
• Specific activity: ≥10 units/mg protein
• Biological source: Staphylococcus aureus

Właściwości

• Nazwa produktu: Endoproteinase Glu-C, Excision Grade, Staphylococcus aureus,
• biological source: Staphylococcus aureus
• Quality Segment: 100
• grade: Proteomics Grade
• form: lyophilized
• specific activity: ≥10 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• technique(s): protein mass spectrometry: suitable
• shipped in: ambient
• storage temp.: 2-8°C

Opis

General description

Native endoproteinase Glu-C from Staphylococcus aureus. Serine protease that specifically hydrolyzes peptide bonds at the carboxylic side of glutamic acid and aspartic acid residues. Inhibited by DFP and α₂-macroglobulin. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.

Other Notes

Houmard, J., and Drapeau, G.R. 1972. Proc. Natl. Acad. Sci. USA69, 3506.

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Z-Phe-Leu-Glu-pNA per min at 25°C, pH 7.8.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Harmful (C)

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08
• Słowo ostrzegawcze: Danger
• Kody zagrożeń: H317,H334
• Ostrzeżenia: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Klasa składowania: 11 - Combustible Solids
• Co się dzieje?: WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable