AP147B
Rabbit Anti-Sheep IgG Antibody, biotin-SP conjugate
Chemicon®, from rabbit
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Informacje o tej pozycji
UNSPSC Code:
12352203
NACRES:
NA.46
eCl@ss:
32160702
Conjugate:
biotin conjugate
Clone:
polyclonal
Application:
ELISA, WB
Citations:
1
Pomoc techniczna
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Pozwól nam pomócbiological source
rabbit
Quality Segment
conjugate
biotin conjugate
antibody form
F(ab′)2 fragment of affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
species reactivity
sheep
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, western blot: suitable
shipped in
wet ice
target post-translational modification
unmodified
Immunogen
Purified Sheep IgG.
Application
DILUTION:
1:50,000 - 1:1,000,000 for ELISA using enzyme-conjugated streptavidin
1:10,000 - 1:100,000 for Western Blots using enzyme-conjugated streptavidin
1:500 - 1:5,000 for enzyme immunohistochemistry on tissue sections
1:200 - 1:1,000 for flow cytometry and fluorescence immunohisto/cytochemistry
Optimal working dilution must be determined by the end user.
1:50,000 - 1:1,000,000 for ELISA using enzyme-conjugated streptavidin
1:10,000 - 1:100,000 for Western Blots using enzyme-conjugated streptavidin
1:500 - 1:5,000 for enzyme immunohistochemistry on tissue sections
1:200 - 1:1,000 for flow cytometry and fluorescence immunohisto/cytochemistry
Optimal working dilution must be determined by the end user.
Rabbit anti-Sheep IgG Antibody, biotin-SP conjugate detects level of Sheep IgG & has been published & validated for use in ELISA & WB.
Research Category
Secondary & Control Antibodies
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies
Whole Immunoglobulin Secondary Antibodies
Biochem/physiol Actions
Based on immunoelectrophoresis, the antibody reacts with the heavy chains on sheep IgG and with light chains common to most sheep immunoglobulins. No antibody was detected against non-immunoglobulin serum protein, but the antibody may cross-react with immunoglobulins from other species.
Physical form
ImmunoAffinity Purified
Lyophilized. Buffer = 0.01M Phosphate buffer, 0.25 M NaCl with 15 mg/mL BSA and 0.05% sodium azide.
Preparation Note
Maintain lyophilized product at 2-8°C for up to 12 months from date of receipt. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles. Expiration date: one year from date of reconstitution.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kody zagrożeń
Ostrzeżenia
Hazard Classifications
Aquatic Chronic 3
Klasa składowania
11 - Combustible Solids
Co się dzieje?
WGK 3
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