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68022

Y. enterocolitica Millichrome™ plus Supplement

suitable for microbiology

Synonim(y):

Y. enterocolitica chromogenic supplement

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Informacje o tej pozycji

NACRES:
NA.74
UNSPSC Code:
41171614
Pomoc techniczna
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sterility

sterile; 0.45 μm filtered

Quality Segment

form

dry powder

shelf life

limited shelf life, expiry date on the label

technique(s)

microbiological culture: suitable

solubility

sterile water: 100 mg/mL

application(s)

food and beverages, microbiology

shipped in

ambient

storage temp.

2-8°C

suitability

selective for bacteria: Yersinia enterocolitica

General description

Y. enterocolitica Millichrome™ plus Supplement is a selective media supplement for the detection of Yersinia enterocolitica. The distinguishing feature of these media is the inclusion of a chromogenic substrate mix that produces visible color changes upon enzymatic activity of Yersinia enterocolitica.Millichrome™ chromogenic media is a specialized type of culture medium used to detect and isolate specific groups of bacteria based on their ability to produce enzymes with chromogenic substrates. The package contains 1 vials sufficient for 1 liter of final media. The prepared supplement is added to 1 liter of melted Y. enterocolitica Millichrome™ plus Agar base cooled at 45-50 °C +/- 2 °C.

Application

Y. enterocolitica Millichrome™ plus Supplement is a selective chromogenic media supplement intended for use in the qualitative detection, differentiation, and presumptive identification of Yersinia enterocolitica from various samples.

Preparation Note

  • Prepare a stock solution of the supplement: Add 100 mg to 1 mL of purified water.
  • Swirl well until complete dissolution. Filter sterilize at 0.45 μm.
  • Warning 2: This supplement stock solution should be used immediately after Preparation, or can be stored at -20 °C and used within 15 days.
  • Add 1 mL of the prepared supplement solution to 1 L of the prepared Y. enterocolitica Millichrome™ plus Agar base cooled at 45-50 °C +/- 2 °C.
  • Swirl gently to homogenize.
  • Pour into sterile Petri dishes.

Analysis Note

The Pathogenic Y. enterocolitica is detected by a characteristic mauve color after only 36-48 h of incubation, while the non-pathogenic Y. enterocolitica and background flora (Citrobacter, Enterobacter, Aeromonas, etc.) form metallic blue colonies or limited growth in metallic blue color after incubation at the same temperature

Other Notes

Composition per vial:
(1 vial is sufficient for 5 L of final media)
Selective mix 0.5g

Legal Information

MilliChrome is a trademark of Merck KGaA, Darmstadt, Germany
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Piktogramy

Health hazard
GHS08

Słowo ostrzegawcze

Danger

Kody zagrożeń

H317,H319,H334

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Sens. 1

Klasa składowania

11 - Combustible Solids

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