27645

Demecolcine

≥98.0% (sum of enantiomers, HPLC)

• Synonim(y): N-Deacetyl-N-methylcolchicine
• Wzór empiryczny (zapis Hilla): C₂₁H₂₅NO₅
• Numer CAS: 477-30-5
• Masa cząsteczkowa: 371.43
• EC Number: 207-514-6
• UNSPSC Code: 12171500
• PubChem Substance ID: 329753007
• Beilstein/REAXYS Number: 2822892
• MDL number: MFCD00075459

Właściwości

• biological source: synthetic
• agency: USP reference standard
• assay: ≥98.0% (sum of enantiomers, HPLC)
• form: powder or crystals
• mp: 184 °C
• storage temp.: 2-8°C
• SMILES string: CN[C@H]1CCc2cc(OC)c(OC)c(OC)c2C3=CC=C(OC)C(=O)C=C13
• InChI: 1S/C21H25NO5/c1-22-15-8-6-12-10-18(25-3)20(26-4)21(27-5)19(12)13-7-9-17(24-2)16(23)11-14(13)15/h7,9-11,15,22H,6,8H2,1-5H3/t15-/m0/s1
• InChI key: NNJPGOLRFBJNIW-HNNXBMFYSA-N

Opis

Biochem/physiol Actions

Often in karyotyping and cell cycle research it is desirable to increase the yield of mitotic cells in a particular phase of the cell cycle. This can be achieved in a variety of ways with the most popular being the use of a cell cycle synchronizing agent such as demecolcine. Demecolcine will arrest cells in metaphase with no remarkable effect on the biochemical events in mitotic cells or in synchronized G₁ and S phase cells. White blood cells are often treated with demecolcine to arrest cells in metaphase.

Other Notes

Binds tubulin hence interfering with microtubule-dependent cell function

Ta strona może zawierać tekst przetłumaczony maszynowo.

Informacja o środkach bezpieczeństwa

• Piktogramy: GHS06
• Słowo ostrzegawcze: Danger
• Kody zagrożeń: H300
• Ostrzeżenia: P264 - P301 + P310
• Hazard Classifications: Acute Tox. 2 Oral
• Klasa składowania: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Co się dzieje?: WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• PPE (środki ochrony indywidualnej): Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges