C8740
CelLytic™ B Cell Lysis Reagent
For bacterial cell lysis, 10× concentrate
Synonim(y):
bacterial cell lysis reagent
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Pozwól nam pomócApplication
A proprietary, non-denaturing formulation of zwitterionic detergents used for the lysis of bacterial cells and extraction of recombinant proteins.
TM[="CelLytic"] B Cell Lysis Reagent has been used for lysing E.coli 5-helix bundle protein. It is also suitable for lysing hepatitis B X-interacting protein (HBXIP) and E. coli TG1 cells.
Features and Benefits
- Higher protein extraction efficiency than traditional methods such as sonication and lysozyme
- Scalable for 1 to 25 grams of bacterial cell paste
- No interference with downstream applications such as affinity chromatography, IP, and Western blotting
- Compatible with protease inhibitors, inhibitor cocktails, chaotropes, salts, chelating agents and reducing agents
Other Notes
10X concentrate of detergent blend contains no buffering components, giving the researcher flexibility to integrate the lysis reagent into their desired system. Also amenable to automation protocols for recombinant protein extraction and purification.
Legal Information
Covered by US Patent No 7,282,475 B2 and are sold for research use only. Commercial use requires addtional licenses.
CelLytic is a trademark of Sigma-Aldrich Co. LLC
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Słowo ostrzegawcze
Warning
Kody zagrożeń
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
Klasa składowania
12 - Non Combustible Liquids
Co się dzieje?
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
PPE (środki ochrony indywidualnej)
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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