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L5542

LB Agar Plates

pre-poured LB agar plates

Synonim(y):

pre-poured LB agar plates

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Rozmiar/SKUDostępnośćCena netto
10 ea
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245,00 zł

Informacje o tej pozycji

NACRES:
NA.74
UNSPSC Code:
41121800
Sterility:
sterile; autoclaved
Suitability:
nonselective for Escherichia coli, nonselective for coliforms

245,00 zł


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grade

Molecular Biology

Quality Segment

sterility

sterile; autoclaved

form

plates (10 cm), plates (10cm, 20-25ml each)

technique(s)

molecular cloning: suitable

capacity

20-25 mL

storage temp.

2-8°C

suitability

nonselective for Escherichia coli, nonselective for coliforms

General description

LB Agar is a highly-referenced microbial growth medium used for the cultivation of Escherichia coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.

Application

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins.
LB Agar Plates has been used:
  • to culture bacteria like, Escherichia coli strain INV- α and methicillin-susceptible Staphylococcus aureus
  • to culture numerous bacteria and is used in strain storage method
  • to analyse the egestion of midgut microbes during sand fly bites

Features and Benefits

LB Agar plates provide:


  • Convenient, ready-to-use 10cm format
  • Standard formulation

Other Notes

1.0% Tryptone


0.5% Yeast Extract


0.5% Sodium Chloride


1.5% Agar
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Ta pozycja
A3340L3027
AC agar suitable for microbiology, NutriSelect® Plus

Millipore

A3340

AC agar

description

pre-poured LB agar plates

description

suitable for microbiology, NutriSelect® Plus

description

Microbiologically tested

sterility

sterile; autoclaved

sterility

non-sterile

sterility

-

suitability

nonselective for Escherichia coli, nonselective for coliforms

suitability

nonselective for coliforms, nonselective for Neisseria spp., nonselective for Staphylococcus spp., nonselective for Escherichia coli, nonselective for Clostridium spp., nonselective for Streptococcus spp., nonselective for bacteria (General Media)

suitability

-

grade

Molecular Biology

grade

-

grade

-

storage temp.

2-8°C

storage temp.

-

storage temp.

-

form

plates (10cm, 20-25ml each), plates (10 cm)

form

powder

form

-

Quality Level

100

Quality Level

200

Quality Level

100


Klasa składowania

13 - Non Combustible Solids

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

PPE (środki ochrony indywidualnej)

Eyeshields, Gloves, type N95 (US)



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Certyfikaty analizy (CoA)

Lot/Batch Number

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Questions

1–5 of 5 Questions  
  1. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  2. Which bacterial culture medium is the best choice for my application?

    1 answer
    1. Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production.  The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.

      Helpful?

  3. What are the differences among the Luria, Lennox and Miller LB formulations?

    1 answer
    1. LB, (originally termed lysogeny broth) was initially composed of tryptone, yeast extract, NaCl and glucose.  Soon after, the glucose was omitted (Miller's version), and later the NaCl content lowered by half (Lennox's version).  For some applications, even lower salt is required (Luria's low salt version).

      Helpful?

  4. Will adding magnesium to the culture medium increase cell density?

    1 answer
    1. In microbial broth formulations that do not already contain magnesium, the addition of 10-20 mM MgCl2 or MgSO4 may increase cell densities.  You may need to also increase the shaking speed of the incubator.

      Helpful?

  5. What is the difference among the many LB - Lennox products?

    1 answer
    1. LB - Lennox is available in many types to suit your needs.  The different product formats include powder, EZMix™ powder, and tablet form.  Each of these forms are available with and without agar for easy LB-agar plate preparation.L3022 (LB Lennox powder)L2897 (LB Lennox powder with agar)L7275 (LB Lennox tablets)L7025 (LB Lennox tablets with agar)L7658 (LB Lennox EZMix(TM) powder)L7533 (LB Lennox EZMix(TM) powder with agar)L5542 (LB Lennox pre-poured agar plates)

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