P4707

Poly-ʟ-Lysine Hydrobromide

synthetic, liquid, 0.01%, suitable for cell culture

• Synonim(y): PLL Solution
• Numer CAS: 25988-63-0
• UNSPSC Code: 12352202
• eCl@ss: 32160406
• NACRES: NA.75
• MDL number: MFCD00165424
• Biological source: synthetic
• Form: liquid
• Technique(s): cell culture | mammalian: suitable
• Concentration: 0.01%
• Mol wt: 70—150 kDa

Właściwości

• Nazwa produktu: Poly-L-lysine solution, 0.01%, sterile-filtered, BioReagent, suitable for cell culture
• biological source: synthetic
• Quality Segment: 300
• sterility: sterile-filtered
• product line: BioReagent
• form: liquid
• mol wt: 70—150 kDa
• packaging: pkg of 50 mL
• concentration: 0.01%
• technique(s): cell culture | mammalian: suitable
• surface coverage: 4 μg/cm²
• impurities: endotoxin, tested
• solubility: water: soluble
• shipped in: ambient
• storage temp.: 2-8°C
• SMILES string: N([C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)O)C(=O)[C@@H](N)CCCCN
• InChI: 1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1
• InChI key: WBSCNDJQPKSPII-KKUMJFAQSA-N

Opis

General description

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Application

Poly-L-lysine polymers can be used in promoting cell adhesion to solid substrates, conjugation to methotrexate for increased drug transport, microencapsulation of islets, cell microencapsulation technology, microarray glass slide coating, and chromosomal preparations. Lower molecular weight poly-L-lysine (30,000-70,000) is less viscuous in solution, but higher molecular weight versions provide more attachment sites per molecule.

• Poly-L-lysine coated glass coverslips have been used for mounting samples for scanning electron microscopy.
• Poly-L-lysine coated wells were used for trapping of PC-12 cells on electrodes.
• Poly-L-lysine-coated borosilicate glass slides were used for the seeding of viral vector-transduced cells.

Biochem/physiol Actions

Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor.

Preparation Note

This product is supplied as a 0.01% sterile filtered solution in water. Coating slides with this solution should be followed with a 5 minute incubation and drying the slides at room temperature. Coated slides will be stable for one year if protected from dust.

Other Notes

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 12 - Non Combustible Liquids
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable