Product P0065 (Phospholipase D) is sold by activity units per milliliter (units/mL). The protein amount is not measured; only the units/mL are provided for this product.
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P0065
Phospholipase D from Streptomyces chromofuscus
≥50,000 units/mL, buffered aqueous glycerol solution
Synonim(y):
Lecithinase D, Phosphatidylcholine phosphatidohydrolase
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
25000 units | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 1120,00 zł |
Informacje o tej pozycji
Numer CAS:
UNSPSC Code:
41106300
NACRES:
NA.77
EC Number:
232-639-8
MDL number:
Numer WE:
Specific activity:
≥50,000 units/mL
1120,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
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Pozwól nam pomócform
buffered aqueous glycerol solution
Quality Segment
specific activity
≥50,000 units/mL
mol wt
~60 kDa
shipped in
dry ice
storage temp.
−70°C
General description
Phospholipase D (phosphatidylcholine cholinehydrolase, EC 3.1.4.4) is a ubiquitous enzyme with a molecular weight of ~ 50,000Da. It is expressed in a variety of plant tissues, few microorganisms and some mammalian tissues.
Application
Phospholipase D from Streptomyces chromofuscus has been used to cleave choline-containing phospholipids (PCs).
Biochem/physiol Actions
Phospholipase D is active on sphingomyelins and lysophospholipids. Phospholipase D hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.
Phospholipase D (PLD) catalyses the hydrolysis of phosphatidylcholines (PC) to yield phosphatidic acid (PA) and choline. PC-PLD activity is regulated by hormones, cytokines, neurotransmitters and growth factors. Thus, it is considered to have a role in signal transduction in various cell types. Three vital lipid messengers produced by PLD plays a vital role in various crucial cellular processes and these lipids also have an essential role in downstream triglyceride and phospholipid synthetic pathway. PLD is essential for exocytosis in neurons and endocrine cells. In addition, it also plays a key role in phagocytosis.
Physical form
Solution in 100 mM Tris/HCl, pH 8.0, 10% glycerol (v/v), and 0.1% Triton X-100 (w/v).
Other Notes
One unit will release 1 μmol of choline from L-α-phosphatidylcholine per hour at pH 8.0 and 30 °C.
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Ta pozycja | |||
|---|---|---|---|
| description ≥50,000 units/mL, buffered aqueous glycerol solution | description lyophilized powder, ≥20 units/mg protein | description ≥200 units/mg protein | description lyophilized powder, ≥1,000 units/mg protein (Lowry) |
| specific activity ≥50,000 units/mL | specific activity ≥20 units/mg protein | specific activity ≥200 units/mg protein | specific activity ≥1,000 units/mg protein (Lowry) |
| form buffered aqueous glycerol solution | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| shipped in dry ice | shipped in - | shipped in - | shipped in - |
| mol wt ~60 kDa | mol wt - | mol wt 23-27 kDa by SDS-PAGE | mol wt - |
| storage temp. −70°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 300 | Quality Level 300 |
Słowo ostrzegawcze
Danger
Kody zagrożeń
Ostrzeżenia
Hazard Classifications
Resp. Sens. 1
Klasa składowania
11 - Combustible Solids
Co się dzieje?
WGK 3
PPE (środki ochrony indywidualnej)
Eyeshields, Gloves, type N95 (US)
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