P2289

Endoproteinase Lys-C from Lysobacter enzymogenes

lyophilized powder

• Numer CAS: 72561-05-8
• UNSPSC Code: 12352204
• EC Number: 276-716-4
• MDL number: MFCD02253020
• Numer WE: 3.4.21.50 (BRENDA, IUBMB)

Właściwości

• form: lyophilized powder
• Quality Segment: 200
• analyte chemical class(es): amino acids
• packaging: vial of ≥3.0 units
• storage temp.: 2-8°C

Opis

General description

Endoproteinase Lys-C is an enzyme that preferentially cleaves at the carboxyl side of lysine residues.

Application

Endoproteinase Lys-C from Lysobacter enzymogenes is useful in the determination of primary structures of proteins. It has been used in a study to investigate the evidence for trisulfide bonds in a recombinant variant of a human IgG2 monoclonal antibody.

Endoproteinase Lys-C has been used for the digestion monoclonal antibodies for disulfide bond assignment.

Biochem/physiol Actions

Endoproteinase Lys-C inactivates the enzyme inositol monophosphatase by cleaving it at a single site directly after Lys 36.

Other Notes

One unit will hydrolyze 1.0 μmole of N-p-tosyl-Gly-Pro-Lys p-nitroanilide per min at pH 7.7 at 25 °C.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08,GHS07
• Słowo ostrzegawcze: Danger
• Narządy docelowe: Respiratory system
• Klasa składowania: 11 - Combustible Solids
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• PPE (środki ochrony indywidualnej): dust mask type N95 (US), Eyeshields, Faceshields, Gloves
• Co się dzieje?: WGK 1
• Kody zagrożeń: H315,H319,H334,H335
• Ostrzeżenia: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3