I bought this kit as replacement of NA0410-1Kt which I was waiting for 1 year and still wasn't able to get due to delay. Plex15 is significantly more expensive, uncomfortable to work with and yields less plasmid DNA. I regret I had to waste my time and money on it.
PLEX15
GenElute™ Endotoxin-free Plasmid Maxiprep Kit
sufficient for 15 purifications
GenElute™ Endotoxin-free Plasmid Maxiprep Kit
Synonim(y):
GenElute™ Endotoxin-free Plasmid Kits, Gen Elute
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Wybierz wielkość
1 KIT
2440,00 zł
2440,00 zł
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Informacje o tej pozycji
NACRES:
NA.52
UNSPSC Code:
41105501
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Pozwól nam pomócNazwa produktu
GenElute™ Endotoxin-free Plasmid Maxiprep Kit, sufficient for 15 purifications
usage
sufficient for 15 purifications
greener alternative product characteristics
Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technique(s)
DNA purification: suitable
greener alternative category
, Aligned
storage temp.
15-25°C
Quality Level
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Ta pozycja | PLX15 | NA0300 | NA0410 |
|---|---|---|---|
| usage sufficient for 15 purifications | usage sufficient for 15 purifications | usage sufficient for 10 purifications | usage sufficient for 25 preparations |
| technique(s) DNA purification: suitable | technique(s) DNA purification: suitable | technique(s) DNA purification: suitable | technique(s) DNA extraction: suitable |
| greener alternative product characteristics Inherently Safer Chemistry for Accident Prevention | greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics Designing Safer Chemicals |
| sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product |
| greener alternative category , Aligned | greener alternative category | greener alternative category | greener alternative category |
| storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C |
Features and Benefits
- High transfection efficiency and yield
- Purify up to 250 μg endotoxin-free plasmid DNA (≤0.1 EU/μg DNA)
- Fast and simple allowing up to 12 preps in less than 2 hours
Application
General description
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
The GenElute Endotoxin-free Plasmid Kit offers a simple, rapid, cost effective method for purifying plasmid DNA with ≤0.1 EU/μg DNA for high efficiency transfection. Endotoxins (also known as lipopolysaccharides or LPS) are often co-purified with plasmid DNA and significantly reduce transfection efficiencies in endotoxin-sensitive cell lines.
Recombinant E. coli is harvested from an overnight culture by centrifugation and subjected to a modified alkaline-SDS lysis procedure to produce a cleared lysate. Endotoxins are removed from the cleared lysate with simple extraction-phase separation steps. Plasmid DNA is further purified by adsorption onto silica in the presence of high salts. After a simple spin-wash step, the bound plasmid DNA is eluted in endotoxin-free water. Up to 250 ug of endotoxin-free plasmid DNA may be prepared from 5-40 mL of culture.
The recovered plasmid DNA is predominately in its supercoiled form. The DNA is ready for immediate use in downstream applications such as transfection, restriction digestion, cloning, sequencing, and PCR amplification.
Recombinant E. coli is harvested from an overnight culture by centrifugation and subjected to a modified alkaline-SDS lysis procedure to produce a cleared lysate. Endotoxins are removed from the cleared lysate with simple extraction-phase separation steps. Plasmid DNA is further purified by adsorption onto silica in the presence of high salts. After a simple spin-wash step, the bound plasmid DNA is eluted in endotoxin-free water. Up to 250 ug of endotoxin-free plasmid DNA may be prepared from 5-40 mL of culture.
The recovered plasmid DNA is predominately in its supercoiled form. The DNA is ready for immediate use in downstream applications such as transfection, restriction digestion, cloning, sequencing, and PCR amplification.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
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Danger
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Central nervous system
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
Klasa składowania
10 - Combustible liquids
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Narzędzia do przygotowywania plazmidów, transkrypcji in vitro, oczyszczania mRNA i formułowania LNP w rozwoju szczepionek i terapii opartych na mRNA.
Tools for plasmid preparation, in vitro transcription, mRNA purification, and LNP formulation in mRNA-based vaccine and therapeutic development.
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