RAB1089

Human TNF-alpha ELISA Kit (for Lysates)

for cell and tissue lysate samples

• Synonim(y): TNF-Alpha
• NACRES: NA.32
• UNSPSC Code: 41116158
• eCl@ss: 32160701

Właściwości

• species reactivity: human
• packaging: kit of 96 wells (12 strips x 8 wells)
• technique(s): ELISA: suitable
• input: sample type tissue lysate; sample type cell lysate
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 30 pg/ml
• detection method: colorimetric
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... TNF(7124)

Opis

General description

Tumor necrosis factor-α (TNF-α) is encoded by the gene mapped to human chromosome 6p21.3, within the major histocompatibility complex (MHC) class III region. The encoded protein belongs to the TNF superfamily.

This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human ANG, CD23, Eotaxin, GCSF, GM-CSF, GRO-alpha, GRO-beta, GRO-gamma, I-309, IFN-gamma, IL-1 alpha, IL-1 beta, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40), IL-12 (p70), IL-15, IL-16, IP-10, MCP-1, MCP-2, MCP-3, MCP-4, MCSF, MIG, MIP-1 alpha, MIP-1 beta, NAP-2, PDGF, PF-4

Application

Please refer to the attached Protocolfor details.

Human tumor necrosis factor-alpha (TNF-α) ELISA Kit has been used in enzyme-linked immunosorbent assay (ELISA) to quantify TNF-α in supernates of human acute lung infection model (ALIM), human umbilical vein endothelial cells (HUVEC) and human microvascular endothelial cells (HMEC-1).

Biochem/physiol Actions

Tumor necrosis factor-α (TNF-α) is a key immunomodulator and proinflammatory cytokine. It is implicated in the immune pathogenesis of various autoimmune, inflammatory diseases and hepatitis B virus (HBV) infection. Mutation in the gene increases the risk of susceptibility to diffuse large B-cell lymphoma (DLBCL) and marginal zone lymphoma. In addition, polymorphism in the gene leads to obsessive-compulsive disorder (OCD) and osteoarthritis (OA).

Other Notes

A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08
• Słowo ostrzegawcze: Danger
• Kody zagrożeń: H319,H334,H412,EUH430
• Ostrzeżenia: P261 - P273 - P305 + P351 + P338 - P391 - P405 - P501
• Klasa składowania: 10 - Combustible liquids
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Hazard Classifications: Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Resp. Sens. 1