The principal of this kit is based on chemical binding and elution rather than size exclusion. To recover more of the small-sized RNA, an increase the amount of Binding Solution is suggested, however some small RNA molecules, including microRNA, siRNA, tRNA, and 5S rRNA, are not efficiently recovered. To maximize the recovery of small RNAs, refer to Protocol A on page 5 of the user guide – link provided below:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/324/136/spectrumrnakitug-ms.pdf
Przejdź do
STRN50
Spectrum™ Plant Total RNA Kit
sufficient for 50 purifications
Cechy związane ze zrównoważonym rozwojem
Synonim(y):
Plant total RNA extraction kit, Plant total RNA isolation kit, Plant total RNA purification kit
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
1 kit | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 1990,00 zł |
Informacje o tej pozycji
NACRES:
NA.52
UNSPSC Code:
41105501
1990,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócusage
sufficient for 50 purifications
Quality Segment
greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Sustainability features
technique(s)
RNA purification: suitable
test parameters
: ~30 min kit run time, sample volume: 100 mg
storage temp.
15-25°C
General description
The Spectrum Plant Total RNA Kit utilizes a lysis and binding chemistry and a convenient column-based ′bind-wash-and-elute′ format to purify up to 100 μg of total RNA from 100 mg of tissue in about 30 minutes. Typical yields range from 20–60 μg.
After grinding tissue to a fine powder in liquid nitrogen, cells are lysed and cellular debris is physically and chemically separated from endogenous RNA. RNA is then bound to a column supported silica substrate and several wash steps remove remaining contaminants.
Total RNA is eluted from the column and used in typical applications, such as Northern Blots, and RT- and qRT-PCR.
After grinding tissue to a fine powder in liquid nitrogen, cells are lysed and cellular debris is physically and chemically separated from endogenous RNA. RNA is then bound to a column supported silica substrate and several wash steps remove remaining contaminants.
Total RNA is eluted from the column and used in typical applications, such as Northern Blots, and RT- and qRT-PCR.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry.
Application
Features and Benefits
- Yields up to 60 μg of pure concentrated RNA per prep
- Efficient protocol allows for RNA purification in 30 min or less
- Specially designed for research with difficult plant tissues
Legal Information
Spectrum is a trademark of Sigma-Aldrich Co. LLC
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Ta pozycja | |||
|---|---|---|---|
| description sufficient for 50 purifications | description sufficient for 250 purifications | description sufficient for 10 purifications | description Suitable for any plant species or tissue |
| usage sufficient for 50 purifications | usage sufficient for 250 purifications | usage sufficient for 10 purifications | usage - |
| technique(s) RNA purification: suitable | technique(s) RNA purification: suitable | technique(s) RNA purification: suitable | technique(s) protein extraction: suitable |
| test parameters : ~30 min kit run time, sample volume: 100 mg | test parameters : ~30 min kit run time, sample volume: 100 mg | test parameters : ~30 min kit run time, sample volume: 100 mg | test parameters - |
| greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics - |
| greener alternative category | greener alternative category | greener alternative category , Aligned | greener alternative category - |
| storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C | storage temp. −20°C |
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Tylko elementy zestawu
Numer produktu
Opis
- Binding Solution
- Lysis Solution
- Wash Solution 1
Elementy zestawu są też dostępne oddzielnie
Numer produktu
Opis
Karta charakterystyki & Cennik
- 2-Mercaptoethanol, Molecular Biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)
Słowo ostrzegawcze
Danger
Kody zagrożeń
Hazard Classifications
Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral
Narządy docelowe
Liver,Heart
Co się dzieje?
WGK 3
Klasa składowania
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
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Which is the exclusion size for RNAs purified with this kit ? I am wondering in the retention size of small RNAs
1 answer-
Helpful?
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What composition of your elution buffer (E8024-50ml) in plant total RNA kit?
1 answer-
The composition of this product is considered proprietary.
Helpful?
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Is it suitable for use with chestnut samples?
Is it suitable for use with chestnut samples?
1 answer-
The kit has been utilized for chestnut samples, as reported in the following paper: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4615883/
Helpful?
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We need to know the elution buffer composition for downstream quantification and sequencing. What solution can be used besides the original elution buffer that can have the same proprerties to be used as a blank?
1 answer-
A suitable solution would be molecular biology-grade water or nuclease-free water. These types of water are typically free of contaminants and nucleases, making them ideal for use as a blank solution in downstream quantification and sequencing experiments.
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