Pomoc techniczna
Potrzebujesz pomocy? Nasz zespół doświadczonych naukowców chętnie Ci pomoże.
Pozwól nam pomócpackaging
pkg of 0.05 mL, pkg of 0.2 mL, pkg of 20 tests, pkg of 5 tests
storage condition
protect from light
color
, Green/Orange/Blue
application(s)
research use
storage temp.
2-8°C
General description
The ZytoLight SPEC RREB1/MYB/CEN 6 Triple Color Probe (PL108) is a direct-labeled FISH probe designed for the qualitative detection of amplifications involving the human RREB1 gene, the human MYB gene, and chromosome 6 alpha satellites in formalin-fixed, paraffin-embedded (FFPE) specimens. It is intended to be used in conjunction with the ZytoLight FISH-Tissue Implementation Kit.
Components:
ZytoLight® SPEC RREB1/MYB/CEN 6 Triple Color Probe comprises:
Fluorescence in situ hybridization (FISH) uses direct-labeled probes (RREB1, MYB, CEN 6) that co-denature with target DNA in pretreated FFPE sections (10 µl/slide, sealed), hybridize overnight (75°C/10 min denaturation, 37°C hybridization), then undergo stringent washing and DAPI counterstaining for visualization via fluorescence microscopy with color-specific filters per ZytoLight FISH-Tissue Implementation Kit.
Components:
ZytoLight® SPEC RREB1/MYB/CEN 6 Triple Color Probe comprises:
- ZyGreen labeled polynucleotides (excitation 503 nm/emission 528 nm), targeting the RREB1 gene region on chromosome 6 (6p24.3).
- ZyRed labeled polynucleotides (excitation 580 nm/emission 599 nm), targeting the MYB gene region on chromosome 6 (6q23.3).
- ZyBlue labeled polynucleotides (excitation 418 nm/emission 467 nm), targeting the alpha satellite centromeric region D6Z1 of chromosome 6.
- Formamide-based hybridization buffer.
Fluorescence in situ hybridization (FISH) uses direct-labeled probes (RREB1, MYB, CEN 6) that co-denature with target DNA in pretreated FFPE sections (10 µl/slide, sealed), hybridize overnight (75°C/10 min denaturation, 37°C hybridization), then undergo stringent washing and DAPI counterstaining for visualization via fluorescence microscopy with color-specific filters per ZytoLight FISH-Tissue Implementation Kit.
Application
The ZytoLight SPEC RREB1/MYB/CEN 6 Triple Color Probe is intended for the qualitative detection of amplifications involving RREB1 and MYB, and for detection of chromosome 6 alpha satellites, in the specified tissue context. It is designed to be used with the ZytoLight FISH-Tissue Implementation Kit.
Analysis Note
In a normal interphase nucleus, two green signals (for RREB1), two red signals (for MYB), and two blue signals (for chromosome 6) are expected. In cases of amplification, an increased number of green or red signals may be observed. Any unexpected signal patterns should be further explored to identify potential genetic rearrangements.
Legal Information
ZytoLight is a registered trademark of ZytoVision GmbH in Germany
Ta strona może zawierać tekst przetłumaczony maszynowo.
Kody zagrożeń
Hazard Classifications
Carc. 2 - Repr. 1B - STOT RE 2 Oral
Narządy docelowe
Blood
Słowo ostrzegawcze
Danger
Klasa składowania
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Wykazy regulacyjne
Wykazy regulacyjne dotyczą głównie produktów chemicznych. Można w nich podawać ograniczoną liczbę informacji na temat produktów niechemicznych. Brak wpisu oznacza, że żaden ze składników nie znajduje się w wykazie. Użytkownik odpowiada za zagwarantowanie bezpiecznego i zgodnego z prawem stosowania produktu.
75-12-7
CAS No.
75-12-7
CAS No.
Wybierz jedną z najnowszych wersji:
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
