Preparation of multifunctional Janus nanoparticles on the basis of SPIONs as targeted drug delivery system.

Passing the Blood-Brain-Barrier (BBB) is a challenging aspect in nanomedicine. Utilizing surfactant particles is reported to be a potent strategy for easier BBB penetration. On the other hand, loading different functional molecules on a single particle is therapeutically and economically beneficial. In this study, multifunctional amphiphilic Janus nanoparticles have been prepared on the basis of superparamagnetic iron oxide nanoparticles. This Janus platform is armed with folic acid targeting agent and Doxorubicin (DOX) drug that have been conjugated on different sides of the nanoparticles. DOX has been conjugated via imine bond that makes these particles pH sensitive. Chemo-physical characters, in-vitro drug release pattern and toxicity of nanoparticles on rat C6 glioma cell line were studied that confirmed the preparation and pH-dependent behavior of nanoparticles. Microscopy observations showed the Janus morphology of nanoparticles and their cell penetration behavior. Prepared Janus nanoparticle can be utilized as a multifunctional nanomedicine platform for brain cancer treatment.