Cellular census of human fibrosis defines functionally distinct stromal cell types and states.

Nature communications (2020-06-04)
Thomas B Layton, Lynn Williams, Fiona McCann, Mingjun Zhang, Marco Fritzsche, Huw Colin-York, Marisa Cabrita, Michael T H Ng, Marc Feldmann, Stephen N Sansom, Dominic Furniss, Weilin Xie, Jagdeep Nanchahal
ABSTRAKT

Fibrotic disorders are some of the most devastating and poorly treated conditions in developed nations, yet effective therapeutics are not identified for many of them. A major barrier for the identification of targets and successful clinical translation is a limited understanding of the human fibrotic microenvironment. Here, we construct a stromal cell atlas of human fibrosis at single cell resolution from patients with Dupuytren's disease, a localized fibrotic condition of the hand. A molecular taxonomy of the fibrotic milieu characterises functionally distinct stromal cell types and states, including a subset of immune regulatory ICAM1+ fibroblasts. In developing fibrosis, myofibroblasts exist along an activation continuum of phenotypically distinct populations. We also show that the tetraspanin CD82 regulates cell cycle progression and can be used as a cell surface marker of myofibroblasts. These findings have important implications for targeting core pathogenic drivers of human fibrosis.

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2-Phenylindole, technical grade, 95%
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Monoclonal Anti-CD140b antibody produced in mouse, clone 18A2, purified immunoglobulin, buffered aqueous solution
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MISSION® esiRNA, targeting human CD82

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