Homogeneous Detection of Trypsin in Protein Mixtures Based on Fluorescence Resonance Energy Transfer between Anionic Conjugated Polymer and Fluorescent Probe.

Herein we describe a novel and simple conjugated polymer-fluorescent probe based platform for trypsin detection from protein mixtures in homogeneous solution. This platform takes advantage of specific interaction between the probe and the active site of trypsin and the electrostatic interaction between the polymer and the protein to mediate energy transfer between the polymer and the probe. This method does not require any separation steps, which should facilitate high-throughput protease screening and drug discovery.