An in vitro gas equilibration method for determination of chemical partition coefficients in fish.

1. A gas equilibration method for the determination of in vitro chemical partition coefficients in mammals was adapted for use with fish. 2. In vitro blood: water and tissue: blood partition coefficients were determined for three chlorinated ethanes in rainbow trout (Oncorhynchus mykiss). 3. In vitro partition coefficients accurately predicted chemical concentrations in tissues of exposed trout.